Summary of RNAseq analysis on CD34+ cell genes that are altered by DWJM, with genes categorized by function
| Overall function . | Gene symbol . | Fold change . | Significance . | Specific gene function . |
|---|---|---|---|---|
| Megakaryocytic differentiation | GATA2 | 2.82 | 3.61e-31 | GATA2 has been shown to be associated with megakaryocytic lineage commitment |
| MEIS1 | 2.51 | 3.37e-07 | MEIS1 expression is associated with lineage commitment toward a megakaryocyte-erythroid progenitor commitment | |
| VWF | 2.63 | 1.24e-07 | VWF expression by flow cytometry is associated with early megakaryocytic differentiation | |
| Clu | 2.35 | 2.55e-07 | Clu messenger RNA is detected in megakaryocytes from the BM, and as plasma glycoprotein, it was found mainly in platelets, suggesting that CLU is produced by megakaryocytes during megakaryocyte development | |
| Selp | 3.10 | 3.24e-09 | Selp also plays a role in late stage of megakaryopoiesis as Selp expression is seen in mature megakaryocytes | |
| ltga2b(CD41) | 1.82 | 2.33e-11 | Ltga2b expression is a marker of definition and primitive hematopoiesis in murine embryo; however, its regulation is also linked to megakaryocytic lineage commitment | |
| Cell mobility, transmigration, and homing | CXCR4 | 1.88 | 7.98e-08 | CXCR4 is known for its important role in homing of HSPC to the BM |
| CXCL8 | 52.65 | 1.08e-245 | CXCL8 was recently found to play a role in HSPC colonization of the sinusoidal endothelial niche and engraftment in zebrafish | |
| HSC markers | ALDH1 | 1.68 | 8.35e-05 | ALDH1 is a stem cell marker as it was found to be overexpressed in HSCs |
| FOS | 7.87 | 6.91e-69 | FOS has been linked to increased HSC repopulation activity. An increase in FOS expression is seen in UCB CD34+ cells cultured in DWJM. | |
| SRGN | −5.14 | 3.68e-83 | SRGN downregulation was found to be associated with undifferentiated human fetal phenotype. |
| Overall function . | Gene symbol . | Fold change . | Significance . | Specific gene function . |
|---|---|---|---|---|
| Megakaryocytic differentiation | GATA2 | 2.82 | 3.61e-31 | GATA2 has been shown to be associated with megakaryocytic lineage commitment |
| MEIS1 | 2.51 | 3.37e-07 | MEIS1 expression is associated with lineage commitment toward a megakaryocyte-erythroid progenitor commitment | |
| VWF | 2.63 | 1.24e-07 | VWF expression by flow cytometry is associated with early megakaryocytic differentiation | |
| Clu | 2.35 | 2.55e-07 | Clu messenger RNA is detected in megakaryocytes from the BM, and as plasma glycoprotein, it was found mainly in platelets, suggesting that CLU is produced by megakaryocytes during megakaryocyte development | |
| Selp | 3.10 | 3.24e-09 | Selp also plays a role in late stage of megakaryopoiesis as Selp expression is seen in mature megakaryocytes | |
| ltga2b(CD41) | 1.82 | 2.33e-11 | Ltga2b expression is a marker of definition and primitive hematopoiesis in murine embryo; however, its regulation is also linked to megakaryocytic lineage commitment | |
| Cell mobility, transmigration, and homing | CXCR4 | 1.88 | 7.98e-08 | CXCR4 is known for its important role in homing of HSPC to the BM |
| CXCL8 | 52.65 | 1.08e-245 | CXCL8 was recently found to play a role in HSPC colonization of the sinusoidal endothelial niche and engraftment in zebrafish | |
| HSC markers | ALDH1 | 1.68 | 8.35e-05 | ALDH1 is a stem cell marker as it was found to be overexpressed in HSCs |
| FOS | 7.87 | 6.91e-69 | FOS has been linked to increased HSC repopulation activity. An increase in FOS expression is seen in UCB CD34+ cells cultured in DWJM. | |
| SRGN | −5.14 | 3.68e-83 | SRGN downregulation was found to be associated with undifferentiated human fetal phenotype. |