Values of IC50 (micromolar) for proTWT, proTCC, proTY93A, and proTΔ154-167 for IgG aPS/PT and IgG aPT-A
| Variable . | proTWT . | proTCC . | proTY93A . | proTΔ154-167 . | Ratio (closed/open) . |
|---|---|---|---|---|---|
| aPS/PT | |||||
| P7 | 71 ± 10 | >80 | 4.5 ± 0.8 | 4.5 ± 0.8 | 18 |
| P13 | >80 | >80 | 4.1 ± 1.1 | 3.5 ± 0.8 | 19 |
| P24 | 22.4 ± 5 | 25 ± 5 | 3.2 ± 0.9 | 2.8 ± 0.8 | 8 |
| aPT-A | |||||
| P7 | 2.6 ± 0.5 | 2.4 ± 0.5 | 2.7 ± 0.4 | 1.2 ± 0.3 | 0.9 |
| P13 | 3.8 ± 1.0 | 4.8 ± 1.1 | 3.7 ± 0.5 | 3.5 ± 0.5 | 1.3 |
| P24 | 1.7 ± 0.3 | 1.5 ± 0.5 | 2.8 ± 0.3 | 2.5 ± 0.8 | 0.5 |
| Variable . | proTWT . | proTCC . | proTY93A . | proTΔ154-167 . | Ratio (closed/open) . |
|---|---|---|---|---|---|
| aPS/PT | |||||
| P7 | 71 ± 10 | >80 | 4.5 ± 0.8 | 4.5 ± 0.8 | 18 |
| P13 | >80 | >80 | 4.1 ± 1.1 | 3.5 ± 0.8 | 19 |
| P24 | 22.4 ± 5 | 25 ± 5 | 3.2 ± 0.9 | 2.8 ± 0.8 | 8 |
| aPT-A | |||||
| P7 | 2.6 ± 0.5 | 2.4 ± 0.5 | 2.7 ± 0.4 | 1.2 ± 0.3 | 0.9 |
| P13 | 3.8 ± 1.0 | 4.8 ± 1.1 | 3.7 ± 0.5 | 3.5 ± 0.5 | 1.3 |
| P24 | 1.7 ± 0.3 | 1.5 ± 0.5 | 2.8 ± 0.3 | 2.5 ± 0.8 | 0.5 |
Each titration was repeated twice, and the values of IC50 were determined by global fit using a simple binding equation (see "Materials and methods"). The ratio was calculated by divining the IC50 values of proTCC (closed) and proTY93A (open).