Comparison of microarray and real-time RT-PCR expression ratios
. | WT inj . | . | D1 . | . | D2 . | . | D3 . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Genes . | Microarray . | PCR . | Microarray . | PCR . | Microarray . | PCR . | Microarray . | PCR . | ||||
| Up-regulated | ||||||||||||
| TdT | 1.0 | 1.1 | 1.5 | 1.1 | 2.0 | 1.7 | 8.6 | 6.1 | ||||
| Sdf1 | 1.1 | 0.9 | 2.0 | 2.5 | 2.1 | 2.8 | 2.6 | 3.7 | ||||
| Spp1 | 0.9 | 0.9 | 2.2 | 3.8 | 1.7 | 4.3 | 2.3 | 6.2 | ||||
| Lef1 | 0.9 | 0.8 | 1.4 | 2.5 | 1.3 | 2.6 | 2.3 | 5.7 | ||||
| Ezh2 | 0.8 | 1.0 | 0.9 | 1.0 | 0.9 | 1.5 | 1.5 | 1.7 | ||||
| Down-regulated | ||||||||||||
| c-fos | 2.7 | 1.0 | 4.9 | 20.0 | 5.6 | 20.0 | 5.6 | 25.0 | ||||
| Sifn1 | 1.0 | 1.4 | 2.5 | 7.1 | 2.7 | 10.0 | 3.1 | 10.0 | ||||
| CD52 | 1.0 | 1.1 | 2.5 | 3.2 | 2.1 | 2.2 | 2.4 | 3.4 | ||||
| Mmp9 | 0.9 | 1.6 | 2.3 | 4.8 | 7.4 | 8.3 | 2.3 | 7.1 | ||||
. | WT inj . | . | D1 . | . | D2 . | . | D3 . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Genes . | Microarray . | PCR . | Microarray . | PCR . | Microarray . | PCR . | Microarray . | PCR . | ||||
| Up-regulated | ||||||||||||
| TdT | 1.0 | 1.1 | 1.5 | 1.1 | 2.0 | 1.7 | 8.6 | 6.1 | ||||
| Sdf1 | 1.1 | 0.9 | 2.0 | 2.5 | 2.1 | 2.8 | 2.6 | 3.7 | ||||
| Spp1 | 0.9 | 0.9 | 2.2 | 3.8 | 1.7 | 4.3 | 2.3 | 6.2 | ||||
| Lef1 | 0.9 | 0.8 | 1.4 | 2.5 | 1.3 | 2.6 | 2.3 | 5.7 | ||||
| Ezh2 | 0.8 | 1.0 | 0.9 | 1.0 | 0.9 | 1.5 | 1.5 | 1.7 | ||||
| Down-regulated | ||||||||||||
| c-fos | 2.7 | 1.0 | 4.9 | 20.0 | 5.6 | 20.0 | 5.6 | 25.0 | ||||
| Sifn1 | 1.0 | 1.4 | 2.5 | 7.1 | 2.7 | 10.0 | 3.1 | 10.0 | ||||
| CD52 | 1.0 | 1.1 | 2.5 | 3.2 | 2.1 | 2.2 | 2.4 | 3.4 | ||||
| Mmp9 | 0.9 | 1.6 | 2.3 | 4.8 | 7.4 | 8.3 | 2.3 | 7.1 | ||||
The ratios, expressed as fold change for both methods, were generated by comparing expression levels in BM of treated animals (D1, D2, D3, or wt injected) to the basal levels in those of wt animals.