Serial in vitro replating assays of E13.5 fetal liver hematopoietic progenitors
. | In vitro passage . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Genotype . | 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | ||||||
| +/+ | 99 ± 23 | 384 ± 86 | 164 ± 41 | 59 ± 76 | 11 ± 9 | 0 | 0 | ||||||
| AML1/EVI1/+ | 162 ± 59 | 388 ± 131 | 365 ± 150 | 403 ± 89 | 436 ± 104 | 407 ± 114 | 626 ± 3 | ||||||
. | In vitro passage . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Genotype . | 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | ||||||
| +/+ | 99 ± 23 | 384 ± 86 | 164 ± 41 | 59 ± 76 | 11 ± 9 | 0 | 0 | ||||||
| AML1/EVI1/+ | 162 ± 59 | 388 ± 131 | 365 ± 150 | 403 ± 89 | 436 ± 104 | 407 ± 114 | 626 ± 3 | ||||||
Cells from methylcellulose culture were disaggregated, washed, and replated at 1 × 104 cells per methylcellulose plate under the same condition every 7 days. Numbers represent colonies per 1 × 104 cells, mean ± SD.