Expressional changes of various hematopoietic regulators in E12.5 AML1/EVI1/+ and AML1−/− fetal liver cells
. | ΔCTKI - ΔCTWT . | Fold (KI/WT) . | ΔCTKO - ΔCTWT . | Fold (KO/WT) . |
|---|---|---|---|---|
| C/EBPA | −1.4 ± 0.1 | 2.7 ± 0.1 | −2.9 ± 0.0 | 7.2 ± 0.1 |
| LMO2 | 3.5 ± 0.3 | 0.1 ± 0.6 | 3.3 ± 0.1 | 0.1 ± 0.3 |
| SCL | 3.9 ± 0.8 | 0.1 ± 1.7 | 2.4 ± 0.2 | 0.2 ± 0.3 |
| c-MYB | −0.5 ± 0.2 | 1.4 ± 1.5 | −2.4 ± 0.3 | 5.4 ± 0.6 |
| GATA1 | −0.4 ± 0.7 | 1.4 ± 1.3 | −1.6 ± 0.5 | 3.0 ± 1.0 |
| PU.1 | −0.4 ± 0.1 | 1.3 ± 0.1 | 8.2 ± 0.1 | 0.0 ± 0.3 |
| NFE2 | 2.9 ± 0.5 | 0.1 ± 1.0 | 0.3 ± 0.2 | 0.6 ± 0.2 |
| CD11b | 1.2 ± 0.4 | 0.4 ± 0.8 | 5.0 ± 1.8 | 0.0 ± 3.6 |
| MCSFR | 0.1 ± 0.2 | 0.9 ± 0.4 | −1.5 ± 0.9 | 2.8 ± 1.8 |
| GCSFR | −0.6 ± 0.1 | 1.5 ± 0.2 | 0.0 ± 0.0 | 1.0 ± 0.0 |
| GMCSFR | 0.1 ± 0.2 | 1.1 ± 0.4 | −0.6 ± 0.1 | 1.5 ± 0.2 |
| MPO | 10 ± 1.1 | 0.0 ± 2.2 | 7.0 ± 0.7 | 0.0 ± 1.3 |
| β-globin | 3.9 ± 0.9 | 0.1 ± 1.7 | 1.0 ± 0.4 | 0.5 ± 0.8 |
| FOG1 | 2.3 ± 0.2 | 0.2 ± 0.3 | 2.0 ± 0.2 | 0.3 ± 0.3 |
| ALASE | 5.6 ± 0.4 | 0.0 ± 0.7 | 1.9 ± 0.4 | 0.3 ± 0.8 |
. | ΔCTKI - ΔCTWT . | Fold (KI/WT) . | ΔCTKO - ΔCTWT . | Fold (KO/WT) . |
|---|---|---|---|---|
| C/EBPA | −1.4 ± 0.1 | 2.7 ± 0.1 | −2.9 ± 0.0 | 7.2 ± 0.1 |
| LMO2 | 3.5 ± 0.3 | 0.1 ± 0.6 | 3.3 ± 0.1 | 0.1 ± 0.3 |
| SCL | 3.9 ± 0.8 | 0.1 ± 1.7 | 2.4 ± 0.2 | 0.2 ± 0.3 |
| c-MYB | −0.5 ± 0.2 | 1.4 ± 1.5 | −2.4 ± 0.3 | 5.4 ± 0.6 |
| GATA1 | −0.4 ± 0.7 | 1.4 ± 1.3 | −1.6 ± 0.5 | 3.0 ± 1.0 |
| PU.1 | −0.4 ± 0.1 | 1.3 ± 0.1 | 8.2 ± 0.1 | 0.0 ± 0.3 |
| NFE2 | 2.9 ± 0.5 | 0.1 ± 1.0 | 0.3 ± 0.2 | 0.6 ± 0.2 |
| CD11b | 1.2 ± 0.4 | 0.4 ± 0.8 | 5.0 ± 1.8 | 0.0 ± 3.6 |
| MCSFR | 0.1 ± 0.2 | 0.9 ± 0.4 | −1.5 ± 0.9 | 2.8 ± 1.8 |
| GCSFR | −0.6 ± 0.1 | 1.5 ± 0.2 | 0.0 ± 0.0 | 1.0 ± 0.0 |
| GMCSFR | 0.1 ± 0.2 | 1.1 ± 0.4 | −0.6 ± 0.1 | 1.5 ± 0.2 |
| MPO | 10 ± 1.1 | 0.0 ± 2.2 | 7.0 ± 0.7 | 0.0 ± 1.3 |
| β-globin | 3.9 ± 0.9 | 0.1 ± 1.7 | 1.0 ± 0.4 | 0.5 ± 0.8 |
| FOG1 | 2.3 ± 0.2 | 0.2 ± 0.3 | 2.0 ± 0.2 | 0.3 ± 0.3 |
| ALASE | 5.6 ± 0.4 | 0.0 ± 0.7 | 1.9 ± 0.4 | 0.3 ± 0.8 |
ΔCT indicates mean CT of indicated gene - mean CT of β-actin; KI, AML1/EVI1 knock-in; KO, AML1 knock-out; fold, fold difference relative to that of WT litters calculated by 2-(ΔCTKI or KO - ΔCTWT). Data are means ± standard deviation.