Binding specificity for the interaction between human PGRP-S and synthetic PGN fragments
. | Association constant Kd (M) . | . | |
|---|---|---|---|
| PGN fragment . | rhPGRP-S . | rdPGRP-LC . | |
| (GlcNAc-MurNAc-l-Ala-d-iso Gln-l-Lys-d-Ala)2 | 3.69 × 10−6 ± 2.65 × 10−6 | NO | |
| (GlcNAc-MurNAc-l-Ala-d-isoGln-l-Lys)2 | 5.50 × 10−8 ± 3.13 × 10−8 | NO | |
| (GlcNAc-MurNAc-l-Ala-d-isoGln)2 | NO | NO | |
| GlcNAc-MurNAc-l-Ala-d-isoGln | NO | NO | |
| MurNAc-l-Ala-d-isoGln-l-Lys | NO | NO | |
| MurNAc-l-Ala-d-isoGln | NO | NO | |
. | Association constant Kd (M) . | . | |
|---|---|---|---|
| PGN fragment . | rhPGRP-S . | rdPGRP-LC . | |
| (GlcNAc-MurNAc-l-Ala-d-iso Gln-l-Lys-d-Ala)2 | 3.69 × 10−6 ± 2.65 × 10−6 | NO | |
| (GlcNAc-MurNAc-l-Ala-d-isoGln-l-Lys)2 | 5.50 × 10−8 ± 3.13 × 10−8 | NO | |
| (GlcNAc-MurNAc-l-Ala-d-isoGln)2 | NO | NO | |
| GlcNAc-MurNAc-l-Ala-d-isoGln | NO | NO | |
| MurNAc-l-Ala-d-isoGln-l-Lys | NO | NO | |
| MurNAc-l-Ala-d-isoGln | NO | NO | |
Each value was determined as described in “Materials and methods” and is expressed as the mean ± SD of 3 independent experiments.
NO indicates Kd value not obtained because of weak binding of the tested PGN fragment at concentrations up to 80 μM.