Absolute cell numbers for TRAP
Time after treatment, h, by treatment type . | Experiment no. 1 . | . | . | . | Experiment no. 2 . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | CD4 (%) . | CD8 (%) . | GR-1 (%) . | B220 (%) . | CD4 (%) . | CD8 (%) . | GR-1 (%) . | B220 (%) . | ||||||
| PBS | ||||||||||||||
| 0 | 960 ± 39 (100) | 261 ± 37 (100) | 400 ± 42 (100) | 403 ± 49 (100) | 221 ± 49 (100) | 61 ± 15 (100) | 99 ± 46 (100) | 213 ± 73 (100) | ||||||
| 1 | 508 ± 149 (53) | 117 ± 19 (46) | 663 ± 83 (167) | 220 ± 66 (56) | 89 ± 38 (41) | 31 ± 9 (54) | 75 ± 15 (85) | 102 ± 36 (48) | ||||||
| 24 | 646 ± 42 (67) | 178 ± 7 (69) | 404 ± 81 (100) | 573 ± 41 (143) | 234 ± 13 (110) | 69 ± 12 (115) | 127 ± 42 (137) | 234 ± 73 (112) | ||||||
| 48 | 765 ± 59 (80) | 226 ± 14 (88) | 385 ± 94 (98) | 510 ± 69 (129) | 218 ± 103 (109) | 44 ± 15 (79) | 117 ± 75 (173) | 183 ± 69 (98) | ||||||
| R-848 | ||||||||||||||
| 0 | 848 ± 77 (100) | 214 ± 35 (100) | 369 ± 139 (100) | 11 ± 44 (100) | 257 ± 47 (100) | 80 ± 16 (100) | 136 ± 68 (100 | 222 ± 89 (100) | ||||||
| 1 | 35 ± 9 (4) | 18 ± 3 (9) | 306 ± 156 (88) | 66 ± 12 (21) | 311 ± 2 (4) | 9 ± 2 (12) | 48 ± 19 (36) | 91 ± 9 (45) | ||||||
| 24 | 195 ± 12 (23) | 29 ± 2 (14) | 151 ± 18 (45) | 349 ± 17 (113) | 52 ± 31 (20) | 11 ± 8 (13) | 21 ± 16 (14) | 37 ± 33 (15) | ||||||
| 48 | 609 ± 108 (72) | 139 ± 12 (66) | 536 ± 73 (172) | 566 ± 179 (190) | 286 ± 82 (110) | 53 ± 16 (65) | 134 ± 23 (108) | 283 ± 100 (129) | ||||||
Time after treatment, h, by treatment type . | Experiment no. 1 . | . | . | . | Experiment no. 2 . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | CD4 (%) . | CD8 (%) . | GR-1 (%) . | B220 (%) . | CD4 (%) . | CD8 (%) . | GR-1 (%) . | B220 (%) . | ||||||
| PBS | ||||||||||||||
| 0 | 960 ± 39 (100) | 261 ± 37 (100) | 400 ± 42 (100) | 403 ± 49 (100) | 221 ± 49 (100) | 61 ± 15 (100) | 99 ± 46 (100) | 213 ± 73 (100) | ||||||
| 1 | 508 ± 149 (53) | 117 ± 19 (46) | 663 ± 83 (167) | 220 ± 66 (56) | 89 ± 38 (41) | 31 ± 9 (54) | 75 ± 15 (85) | 102 ± 36 (48) | ||||||
| 24 | 646 ± 42 (67) | 178 ± 7 (69) | 404 ± 81 (100) | 573 ± 41 (143) | 234 ± 13 (110) | 69 ± 12 (115) | 127 ± 42 (137) | 234 ± 73 (112) | ||||||
| 48 | 765 ± 59 (80) | 226 ± 14 (88) | 385 ± 94 (98) | 510 ± 69 (129) | 218 ± 103 (109) | 44 ± 15 (79) | 117 ± 75 (173) | 183 ± 69 (98) | ||||||
| R-848 | ||||||||||||||
| 0 | 848 ± 77 (100) | 214 ± 35 (100) | 369 ± 139 (100) | 11 ± 44 (100) | 257 ± 47 (100) | 80 ± 16 (100) | 136 ± 68 (100 | 222 ± 89 (100) | ||||||
| 1 | 35 ± 9 (4) | 18 ± 3 (9) | 306 ± 156 (88) | 66 ± 12 (21) | 311 ± 2 (4) | 9 ± 2 (12) | 48 ± 19 (36) | 91 ± 9 (45) | ||||||
| 24 | 195 ± 12 (23) | 29 ± 2 (14) | 151 ± 18 (45) | 349 ± 17 (113) | 52 ± 31 (20) | 11 ± 8 (13) | 21 ± 16 (14) | 37 ± 33 (15) | ||||||
| 48 | 609 ± 108 (72) | 139 ± 12 (66) | 536 ± 73 (172) | 566 ± 179 (190) | 286 ± 82 (110) | 53 ± 16 (65) | 134 ± 23 (108) | 283 ± 100 (129) | ||||||
Zero hours after treatment indicates pretreatment count. Plus/minus values indicate absolute number of cells/μL blood in animals undergoing treatment either with PBS or R-848. Numbers of animals in each treatment category: experiment no. 1: PBS, n. = 3; R-848, n = 3; experiment no. 2: PBS, n = 3; R-848, n = 2. Data are based on 5 (PBS, 6) animals measured individually in 2 separate experiments. Values are presented as means ± SD of all animals measured in each experiment.