Primer and probe combinations used for quantitative real-time PCR
Target gene . | Oligonucleotide sequence . |
|---|---|
| FLT3 | |
| Forward | 5′-AGC ATC CCA GTC AAT CAG-3′ |
| Reverse | 5′-CTG GCT GGT GCT TAT GA-3′ |
| Probe | 5′- (FAM) -TTA AAG CCT ACC CAC AAA TCA GAT GT- (TAMRA) -3′ |
| FLT3L | |
| Forward | 5′-GAG CCC AAC AAC CTA TCT C-3′ |
| Reverse | 5′-GGA CGA AGC GAA GAC A-3′ |
| Probe | 5′- (FAM) -ATG GAG CGG CTC AAG ACT GT- (TAMRA) -3′ |
| GAPDH | |
| Forward | 5′-GTC GGA GTC AAC GGA TT-3′ |
| Reverse | 5′-AAG CTT CCC GTT CTC AG-3′ |
| Probe | 5′- (FAM) -TCA ACT ACA TGG TTT ACA TGT TCC AA- (TAMRA) -3′ |
Target gene . | Oligonucleotide sequence . |
|---|---|
| FLT3 | |
| Forward | 5′-AGC ATC CCA GTC AAT CAG-3′ |
| Reverse | 5′-CTG GCT GGT GCT TAT GA-3′ |
| Probe | 5′- (FAM) -TTA AAG CCT ACC CAC AAA TCA GAT GT- (TAMRA) -3′ |
| FLT3L | |
| Forward | 5′-GAG CCC AAC AAC CTA TCT C-3′ |
| Reverse | 5′-GGA CGA AGC GAA GAC A-3′ |
| Probe | 5′- (FAM) -ATG GAG CGG CTC AAG ACT GT- (TAMRA) -3′ |
| GAPDH | |
| Forward | 5′-GTC GGA GTC AAC GGA TT-3′ |
| Reverse | 5′-AAG CTT CCC GTT CTC AG-3′ |
| Probe | 5′- (FAM) -TCA ACT ACA TGG TTT ACA TGT TCC AA- (TAMRA) -3′ |
FAM indicates 6-carboxyfluorescein; TAMRA, 6-carboxytetramethyl-rhodamine.