Name of locus . | Forward primer . | Taqman probe . | Reverse primer . |
|---|---|---|---|
| Mouse Ey | CAAGCTACATGTGGATCCTGAGAA | TCAAACTCTTGGGTAATGTGCTGGTGATTG | TGCCGAAGTGACTAGCCAAA |
| Mouse βhl | AGGCAGCTATCACAAGCATCTG | AGAAACTCTGGGAAGGCTCCTGATTGTTTACC | AACTTGTCAAAGAATCTCTGAGTCCAT |
| Mouse βmaj + βmin | GTGAGCTCCACTGTGACAAGCT | CATGTGGATCCTGAGAACTTCAGGCTCCT | GGTGGCCCAGCACAATCACGATC |
| Human ϵ | GCCTTTGCTAAGCTGAGTGAG | TCAAGCTCCTGGGTAACGTGATGGTGA | TTGCCAAAGTGAGTAGCCAGAA |
| Human γG + γA | GTGGAAGATGCTGGAGGAGAAA | AGGCTCCTGGTTGTCTACCCATGGACC | TGCCATGTGCCTTGACTTTG |
| Human β | GCAAGGTGAACGTGGATGAAGT | CAGGCTGCTGGTGGTCTACCCTTGGACCC | TAACAGCATCAGGAGTGGACAGA |
| Mouse ζ | GCGAGCTGCATGCCTACAT | TGGATCCGGTCAACTTCAAGCTCCTGT | GCCATTGTGACCAGCAGACA |
| Mouse GPA | GCCGAATGACAAAGAAAAGTTCA | TTGACATCCAATCTCCTGAGGGTGGTGA | TCAATAGAACTCAAAGGCACACTGT |
Name of locus . | Forward primer . | Taqman probe . | Reverse primer . |
|---|---|---|---|
| Mouse Ey | CAAGCTACATGTGGATCCTGAGAA | TCAAACTCTTGGGTAATGTGCTGGTGATTG | TGCCGAAGTGACTAGCCAAA |
| Mouse βhl | AGGCAGCTATCACAAGCATCTG | AGAAACTCTGGGAAGGCTCCTGATTGTTTACC | AACTTGTCAAAGAATCTCTGAGTCCAT |
| Mouse βmaj + βmin | GTGAGCTCCACTGTGACAAGCT | CATGTGGATCCTGAGAACTTCAGGCTCCT | GGTGGCCCAGCACAATCACGATC |
| Human ϵ | GCCTTTGCTAAGCTGAGTGAG | TCAAGCTCCTGGGTAACGTGATGGTGA | TTGCCAAAGTGAGTAGCCAGAA |
| Human γG + γA | GTGGAAGATGCTGGAGGAGAAA | AGGCTCCTGGTTGTCTACCCATGGACC | TGCCATGTGCCTTGACTTTG |
| Human β | GCAAGGTGAACGTGGATGAAGT | CAGGCTGCTGGTGGTCTACCCTTGGACCC | TAACAGCATCAGGAGTGGACAGA |
| Mouse ζ | GCGAGCTGCATGCCTACAT | TGGATCCGGTCAACTTCAAGCTCCTGT | GCCATTGTGACCAGCAGACA |
| Mouse GPA | GCCGAATGACAAAGAAAAGTTCA | TTGACATCCAATCTCCTGAGGGTGGTGA | TCAATAGAACTCAAAGGCACACTGT |
All Taqman probes were labeled with FAM (6-carboxyfluorescein) and TAMRA (6-carboxy-tetramethylrhodamine).