Primer pair sequences with annealing temperature and product size
Primer pairs (5′-3′) . | Position . | Annealing temperature, °C . | Product, bp . |
|---|---|---|---|
| Runx2/Cbfa1 | 64 | 270 | |
| CCCCACGACAACCGCACCAT | FOW | ||
| CACTCCGGCCCACAAATC | REV | ||
| Osteocalcin | 56 | 235 | |
| GTAGTGAAGAGACCCAGGCG | FOW | ||
| GGGAAGAGGAAAGAAGGGTG | REV | ||
| Collagen I | 60 | 537 | |
| GCAACATGGAGACTGGTGAG | FOW | ||
| GCAACATGGAGACTGGTGAG | REV | ||
| Alkaline phosphatase | 56 | 475 | |
| ACGTGGCTAAGAATGTCATC | FOW | ||
| CTGGTAGGCGATGTCCTTA | REV | ||
| DKK1 | 58 | 185 | |
| GGTATTCCAGAAGAACCACC | FOW | ||
| GAGAGCCTTTTCTCCTATGC | REV | ||
| sFRP1 | 60 | 616 | |
| TCTACACCAAGCCACCTCAG | FOW | ||
| CAGTCACCCCATTCTTCAGG | REV | ||
| sFRP2 | 57 | 386 | |
| AGTTCCTGTGCTCGCTCTTC | FOW | ||
| AATGGTCTTGCTCTTGGTCTC | REV | ||
| sFRP3 | 60 | 701 | |
| ACATGACTAAGATGCCCAACCAC | FOW | ||
| GAGTGCATCCCTCACACTTCTCAG | REV | ||
| sFRP4 | 69 | 826 | |
| AACATCACGCGGATGCCCAACCA | FOW | ||
| GATTACTACGACTGGTGCGCCCG | REV | ||
| Noggin | 65 | 289 | |
| AGAAGCTGCGGAGGAAGTTACAG | FOW | ||
| GAGTTCTAGCACGAGCACTTGCA | REV | ||
| Gremlin | 55 | 285 | |
| ACAGTATGAGCCGCACAG | FOW | ||
| ACCAGTCTCGCTTCAGGTAT | REV | ||
| Interleukin-7 | 66 | 429 | |
| TTTTATTCCGTGCTGCTCGC | FOW | ||
| GCCCTAATCCGTTTTGACCA | REV | ||
| Interleukin-7 receptor | 55 | 428 | |
| GAAGGTTGGAGAAAAGAGTC | FOW | ||
| CAAAAATGCTGATGGTTAGTAAG | REV | ||
| β2-microglobulin | 63 | 334 | |
| CTCGCGCTACTCTCTTCTCTTTCTGG | FOW | ||
| GCTTACATGTCTCGATCCCACTTAA | REV |
Primer pairs (5′-3′) . | Position . | Annealing temperature, °C . | Product, bp . |
|---|---|---|---|
| Runx2/Cbfa1 | 64 | 270 | |
| CCCCACGACAACCGCACCAT | FOW | ||
| CACTCCGGCCCACAAATC | REV | ||
| Osteocalcin | 56 | 235 | |
| GTAGTGAAGAGACCCAGGCG | FOW | ||
| GGGAAGAGGAAAGAAGGGTG | REV | ||
| Collagen I | 60 | 537 | |
| GCAACATGGAGACTGGTGAG | FOW | ||
| GCAACATGGAGACTGGTGAG | REV | ||
| Alkaline phosphatase | 56 | 475 | |
| ACGTGGCTAAGAATGTCATC | FOW | ||
| CTGGTAGGCGATGTCCTTA | REV | ||
| DKK1 | 58 | 185 | |
| GGTATTCCAGAAGAACCACC | FOW | ||
| GAGAGCCTTTTCTCCTATGC | REV | ||
| sFRP1 | 60 | 616 | |
| TCTACACCAAGCCACCTCAG | FOW | ||
| CAGTCACCCCATTCTTCAGG | REV | ||
| sFRP2 | 57 | 386 | |
| AGTTCCTGTGCTCGCTCTTC | FOW | ||
| AATGGTCTTGCTCTTGGTCTC | REV | ||
| sFRP3 | 60 | 701 | |
| ACATGACTAAGATGCCCAACCAC | FOW | ||
| GAGTGCATCCCTCACACTTCTCAG | REV | ||
| sFRP4 | 69 | 826 | |
| AACATCACGCGGATGCCCAACCA | FOW | ||
| GATTACTACGACTGGTGCGCCCG | REV | ||
| Noggin | 65 | 289 | |
| AGAAGCTGCGGAGGAAGTTACAG | FOW | ||
| GAGTTCTAGCACGAGCACTTGCA | REV | ||
| Gremlin | 55 | 285 | |
| ACAGTATGAGCCGCACAG | FOW | ||
| ACCAGTCTCGCTTCAGGTAT | REV | ||
| Interleukin-7 | 66 | 429 | |
| TTTTATTCCGTGCTGCTCGC | FOW | ||
| GCCCTAATCCGTTTTGACCA | REV | ||
| Interleukin-7 receptor | 55 | 428 | |
| GAAGGTTGGAGAAAAGAGTC | FOW | ||
| CAAAAATGCTGATGGTTAGTAAG | REV | ||
| β2-microglobulin | 63 | 334 | |
| CTCGCGCTACTCTCTTCTCTTTCTGG | FOW | ||
| GCTTACATGTCTCGATCCCACTTAA | REV |
FOW indicates forward; REV, reverse.