Phenotypic characteristics of freshly isolated CD14+/VEGFR-2– cells and CD14+/VEGFR-2+ cells from peripheral blood
Antibody . | CD14+/VEGFR-2+ cells, % . | CD14+/VEGFR-2– cells, % . | HAECs, % . |
|---|---|---|---|
| Stem/progenitor/mature endothelial cell markers | |||
| VEGFR-2 | 100 | 0.005 ± 0.001 | 5 |
| VEGFR-1 | 78 ± 1.5 | 81 ± 1.33 | 3 |
| Tie-2 | 100 | 23 ± 2.5 | 0 |
| CD34 | 3 ± 2.1 | 0.01 ± 0.007 | 2 |
| CD123 | 0 | 28 ± 2.1 | 0 |
| CD133 | 2 ± 0.6 | 0.05 ± 0.002 | 0 |
| CD90 | 0 | 0 | 0 |
| AcLDL | 100 | 100 | 100 |
| CD31 | 100 | 20 ± 2.6 | 100 |
| CD144 | 0.2 ± 0.01 | 0 | 100 |
| VWF | 100 | 0 | 100 |
| Ulex europaeus | 100 | 100 | 100 |
| Monocyte/macrophage markers | |||
| CD14 | 98 ± 0.2 | 97 ± 2.2 | 0 |
| CD15 | 9 ± 0.02 | 8 ± 3.2 | 0 |
| CD11b | 95 ± 7.2 | 92 ± 2.4 | 0 |
| CD11c | 6 ± 5.6 | 84 ± 2.7 | 0 |
| CD68 | 0 | 3 ± 1.5 | 0 |
| CD83 | 0 | 2.7 ± 1.4 | 0 |
| Control markers | |||
| α-actin | 0 | 0 | 0 |
| Fibroblasts | 0 | 0 | 0 |
| CD45 | 78 ± 10.1 | 100 | 0 |
| CD3 | 0 | 0 | 0 |
| CD56 | 0 | 0 | 0 |
| CD16 | 95 ± 4.8 | 92 ± 0.4 | 0 |
| CD19 | 0 | 0 | 0 |
Antibody . | CD14+/VEGFR-2+ cells, % . | CD14+/VEGFR-2– cells, % . | HAECs, % . |
|---|---|---|---|
| Stem/progenitor/mature endothelial cell markers | |||
| VEGFR-2 | 100 | 0.005 ± 0.001 | 5 |
| VEGFR-1 | 78 ± 1.5 | 81 ± 1.33 | 3 |
| Tie-2 | 100 | 23 ± 2.5 | 0 |
| CD34 | 3 ± 2.1 | 0.01 ± 0.007 | 2 |
| CD123 | 0 | 28 ± 2.1 | 0 |
| CD133 | 2 ± 0.6 | 0.05 ± 0.002 | 0 |
| CD90 | 0 | 0 | 0 |
| AcLDL | 100 | 100 | 100 |
| CD31 | 100 | 20 ± 2.6 | 100 |
| CD144 | 0.2 ± 0.01 | 0 | 100 |
| VWF | 100 | 0 | 100 |
| Ulex europaeus | 100 | 100 | 100 |
| Monocyte/macrophage markers | |||
| CD14 | 98 ± 0.2 | 97 ± 2.2 | 0 |
| CD15 | 9 ± 0.02 | 8 ± 3.2 | 0 |
| CD11b | 95 ± 7.2 | 92 ± 2.4 | 0 |
| CD11c | 6 ± 5.6 | 84 ± 2.7 | 0 |
| CD68 | 0 | 3 ± 1.5 | 0 |
| CD83 | 0 | 2.7 ± 1.4 | 0 |
| Control markers | |||
| α-actin | 0 | 0 | 0 |
| Fibroblasts | 0 | 0 | 0 |
| CD45 | 78 ± 10.1 | 100 | 0 |
| CD3 | 0 | 0 | 0 |
| CD56 | 0 | 0 | 0 |
| CD16 | 95 ± 4.8 | 92 ± 0.4 | 0 |
| CD19 | 0 | 0 | 0 |
Data are expressed as mean ± SD. n = 5 CD14+/VEGFR-2+ cells; n = 5 CD14+/VEGFR-2–2 cells. HAECs were the primary cell line.