Primers for LMO2 distal and proximal promoter constructs
Position (mutation) . | Sequence (5′-3′) . |
|---|---|
| -23025 | ccgctcgagGGAGAAGTAAATACAGGCTG |
| -22076 | cccaagcttGAGTGGTCTCCCTTTGTGG |
| -661 | ccgctcgagGGACCGGGCAGCTGTCTCT |
| -661 (E box) | ccgctcgagGGACCGGGAAGCTATCTCTTTAAATGT |
| -661 (i) | ccgctcgagGGACCGGGCAGCTGTCTCTACAAATGTGATTTC |
| -661 (Ets1) | ccgctcgagGACCGGGCAGCTGTCTCTTTAAATGTGATTTCTTCTATTGTATTTG |
| -661 (ii) | ccgctcgagGGACCGGGCAGCTGTCTCTTTAAATGTGATTTCCTTCTAATATATTTG |
| -582 | ccgctcgagGAGACAGAGGGAAGCTGAGCG |
| -572 | cccaagcttCCCTCTGTCTCTGGTTTCATTT |
| -572 (iii) | cccaagcttCCCTCTGTCTCTGGTTTCATTTCCTTTTTCCTGATAATGATTCAAA |
| -572 (Ets2) | cccaagcttCCCTCTGTCTCTGGTTTCATTTCCTTTTTCTTGATCACGATTC |
| -572 (Ets3) | cccaagcttCCCTCTGTCTCTGGTTTCATTTCTTTTTTCCTGATCACGATTC |
| -572 (Ets2,3) | cccaagcttCCCTCTGTCTCTGGTTTCATTTCTTTTTTCTTGATCACGATTC |
| -572 (iv) | cccaagcttCCCTCTGTCTTTTGTTTCATTTCCTTTTTCCTGATCACGATTC |
| -572 (v) | cccaagcttCACTATGTCTCTGGTTTCATTTCCTTTTTCCTGATCACGATTC |
| -313 | cccaagcttCAGGACTTAACCTTCCATCCC |
Position (mutation) . | Sequence (5′-3′) . |
|---|---|
| -23025 | ccgctcgagGGAGAAGTAAATACAGGCTG |
| -22076 | cccaagcttGAGTGGTCTCCCTTTGTGG |
| -661 | ccgctcgagGGACCGGGCAGCTGTCTCT |
| -661 (E box) | ccgctcgagGGACCGGGAAGCTATCTCTTTAAATGT |
| -661 (i) | ccgctcgagGGACCGGGCAGCTGTCTCTACAAATGTGATTTC |
| -661 (Ets1) | ccgctcgagGACCGGGCAGCTGTCTCTTTAAATGTGATTTCTTCTATTGTATTTG |
| -661 (ii) | ccgctcgagGGACCGGGCAGCTGTCTCTTTAAATGTGATTTCCTTCTAATATATTTG |
| -582 | ccgctcgagGAGACAGAGGGAAGCTGAGCG |
| -572 | cccaagcttCCCTCTGTCTCTGGTTTCATTT |
| -572 (iii) | cccaagcttCCCTCTGTCTCTGGTTTCATTTCCTTTTTCCTGATAATGATTCAAA |
| -572 (Ets2) | cccaagcttCCCTCTGTCTCTGGTTTCATTTCCTTTTTCTTGATCACGATTC |
| -572 (Ets3) | cccaagcttCCCTCTGTCTCTGGTTTCATTTCTTTTTTCCTGATCACGATTC |
| -572 (Ets2,3) | cccaagcttCCCTCTGTCTCTGGTTTCATTTCTTTTTTCTTGATCACGATTC |
| -572 (iv) | cccaagcttCCCTCTGTCTTTTGTTTCATTTCCTTTTTCCTGATCACGATTC |
| -572 (v) | cccaagcttCACTATGTCTCTGGTTTCATTTCCTTTTTCCTGATCACGATTC |
| -313 | cccaagcttCAGGACTTAACCTTCCATCCC |
The linker sequence to facilitate cloning is shown in lowercase, and the mutated sequence is underlined.