Table 2.

Clotting time versus γ′ peptide concentration


γ′ Peptide, μM

Thrombin time, sec
0   18.6  
20   26.6  
40   31.0  
80
 
62.4
 

γ′ Peptide, μM

Thrombin time, sec
0   18.6  
20   26.6  
40   31.0  
80
 
62.4
 

Measurements contained 250 nM fibrinogen, 2 nM thrombin, and the indicated amount of γ′ peptide in 5 mM Tris, 145 mM NaCl, 0.1% PEG, pH 7.4. Reaction was initiated by adding thrombin and incubating at 37°C.

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