Synthetic oligonucleotides used in the experiments
Oligonucleotides . | Sequence . | Method . |
|---|---|---|
| CD5 1 sense (intron) | 5′-GTGAAGGGCTGCTTACTGCACACAGC-3′ | RT-PCR |
| CD5 2 sense (U3 HERV) | 5′-AGTGGGTCTAAGGGAGGAGACCAC-3′ | RT-PCR |
| CD5 3 sense E1B (leader HERV) | 5′-AGTCCGGACAACTGAAACTTGTTCGCT-3′ | RT-PCR and quantitative PCR |
| CD5 4 sense (gag HERV) | 5′-CGCTTGACAGGACTGGTCTTGGGA-3′ | RT-PCR |
| CD5 5 sense (promotor E1A) | 5′-GATGCATGGCCTTGTCCTGTGTG-3′ | RT-PCR |
| CD5 6 sense E1A | 5′-GAAGGCCAGAAACCATGCCCATGG-3′ | RT-PCR and quantitative PCR |
| CD5 7 sense (intron) | 5′-ATGTGTGTGAGTACTGTGTGTAGGGGA-3′ | RT-PCR |
| CD5 8 sense (intron) | 5′-ATGTGTGTGAGTACTGTGTGGGGGGA-3′ | RT-PCR |
| CD5 9 sense E2 | 5′-TCGGACGGCTCAGCTGGTATGAC-3′ | RT-PCR and quantitative PCR |
| CD5 E5-6 antisense | 5′-TGGAAACCTGAGCAAAGGAGGGCA-3′ | RT-PCR |
| CD5 E3 antisense | 5′-TGCCATCCGTCCTTGAGGTAGAC-3′ | Quantitative PCR |
| CD5 E1A antisense | 5′-CTGGCCGGGTATCTCGCTCTCAGAGAGGGA-3′ | In situ hybridization |
| CD5 E1A sense | 5′-TCCCTCTCTGAGAGCGAGATACCCGGCCAG-3′ | In situ hybridization |
| CD5 E1B antisense | 5′-ATCCTCCACAGGGGCCTGCCATGCACTGCT-3′ | In situ hybridization |
| CD5 E1B sense | 5′-AGCAGTGCATGGCAGGCCCCTGTGGAGGAT-3′ | In situ hybridization |
| CD5 E1B sense | 5′-GGTGTCTGAGGGGTTTTGTCTGTG-3′ | Cloning |
| CD5 E11 antisense | 5′-CGGGGTAGAAATAGGGACATGATTC-3′ | Cloning |
| CD5 E10 antisense | 5′-CATGGATCCCAGAATTCGCAGCCTCTG-3′ | Cloning |
| CD3ζ sense | 5′-TGCTGGATGGAATCCTCTT-3′ | RT-PCR |
| CD3ζ antisense | 5′-GTCTGGCCTTTGAGTGGTGA-3′ | RT-PCR |
| CD79b sense | 5′-AGAAGTGCAACAACACCTCGGAGGTCTACC-3′ | RT-PCR |
| CD79b antisense | 5′-CCATCCCATGTGTGGGGACGGATC-3′ | RT-PCR |
| GAPDH sense | 5′-GAAGGTGAAGGTCGGAGTC-3′ | RT-PCR and quantitative PCR |
| GAPDH antisense | 5′-GAAGATGGTGATGGGATTTC-3′ | RT-PCR and quantitative PCR |
Oligonucleotides . | Sequence . | Method . |
|---|---|---|
| CD5 1 sense (intron) | 5′-GTGAAGGGCTGCTTACTGCACACAGC-3′ | RT-PCR |
| CD5 2 sense (U3 HERV) | 5′-AGTGGGTCTAAGGGAGGAGACCAC-3′ | RT-PCR |
| CD5 3 sense E1B (leader HERV) | 5′-AGTCCGGACAACTGAAACTTGTTCGCT-3′ | RT-PCR and quantitative PCR |
| CD5 4 sense (gag HERV) | 5′-CGCTTGACAGGACTGGTCTTGGGA-3′ | RT-PCR |
| CD5 5 sense (promotor E1A) | 5′-GATGCATGGCCTTGTCCTGTGTG-3′ | RT-PCR |
| CD5 6 sense E1A | 5′-GAAGGCCAGAAACCATGCCCATGG-3′ | RT-PCR and quantitative PCR |
| CD5 7 sense (intron) | 5′-ATGTGTGTGAGTACTGTGTGTAGGGGA-3′ | RT-PCR |
| CD5 8 sense (intron) | 5′-ATGTGTGTGAGTACTGTGTGGGGGGA-3′ | RT-PCR |
| CD5 9 sense E2 | 5′-TCGGACGGCTCAGCTGGTATGAC-3′ | RT-PCR and quantitative PCR |
| CD5 E5-6 antisense | 5′-TGGAAACCTGAGCAAAGGAGGGCA-3′ | RT-PCR |
| CD5 E3 antisense | 5′-TGCCATCCGTCCTTGAGGTAGAC-3′ | Quantitative PCR |
| CD5 E1A antisense | 5′-CTGGCCGGGTATCTCGCTCTCAGAGAGGGA-3′ | In situ hybridization |
| CD5 E1A sense | 5′-TCCCTCTCTGAGAGCGAGATACCCGGCCAG-3′ | In situ hybridization |
| CD5 E1B antisense | 5′-ATCCTCCACAGGGGCCTGCCATGCACTGCT-3′ | In situ hybridization |
| CD5 E1B sense | 5′-AGCAGTGCATGGCAGGCCCCTGTGGAGGAT-3′ | In situ hybridization |
| CD5 E1B sense | 5′-GGTGTCTGAGGGGTTTTGTCTGTG-3′ | Cloning |
| CD5 E11 antisense | 5′-CGGGGTAGAAATAGGGACATGATTC-3′ | Cloning |
| CD5 E10 antisense | 5′-CATGGATCCCAGAATTCGCAGCCTCTG-3′ | Cloning |
| CD3ζ sense | 5′-TGCTGGATGGAATCCTCTT-3′ | RT-PCR |
| CD3ζ antisense | 5′-GTCTGGCCTTTGAGTGGTGA-3′ | RT-PCR |
| CD79b sense | 5′-AGAAGTGCAACAACACCTCGGAGGTCTACC-3′ | RT-PCR |
| CD79b antisense | 5′-CCATCCCATGTGTGGGGACGGATC-3′ | RT-PCR |
| GAPDH sense | 5′-GAAGGTGAAGGTCGGAGTC-3′ | RT-PCR and quantitative PCR |
| GAPDH antisense | 5′-GAAGATGGTGATGGGATTTC-3′ | RT-PCR and quantitative PCR |