UCN-01 inhibits G2 arrest induced by tachpyridine
Treatment . | Cells in G2/M, % . |
|---|---|
| Untreated | 21 ± 3 |
| 100 nM UCN-01 | 18 ± 5 |
| 10 μM tachpyridine | 43 ± 1 |
| 100 nM UCN-01 + 10 μM tachpyridine | 26 ± 3 |
Treatment . | Cells in G2/M, % . |
|---|---|
| Untreated | 21 ± 3 |
| 100 nM UCN-01 | 18 ± 5 |
| 10 μM tachpyridine | 43 ± 1 |
| 100 nM UCN-01 + 10 μM tachpyridine | 26 ± 3 |
HCT 116 cells were either left untreated, treated with 100 nM UCN-01 for 24 hours and 45 minutes, treated with 10 μM tachpyridine for 24 hours, or pretreated for 45 minutes with 100 nM UCN-01 followed by the addition of 10 μM tachpyridine for 24 hours. Cells were analyzed by flow cytometry. Shown are the means and standard deviations of 3 independent experiments.