Percentage of successful MAPC cultures obtained from primary and secondary BM recipients and age-matched controls
. | 1st recipient 1 month after transplantation, n = 4 . | 1st recipient 2 months after transplantation, n = 4 . | 1st recipient 3 months after transplantation, n = 4 . | 2nd recipient 2 months after transplantation, n = 9 . |
|---|---|---|---|---|
| Successful MAPC cx in age-matched mice | 2-mo-old mice 4/6, 66% | 3-mo-old mice 5/11, 45% | 4-mo-old mice 3/7, 42% | 5- to 6-mo-old mice 1/10, 10% |
| Successful MAPC cx in transplant recipients | n = 3, 75% | n = 2, 50% | n = 2, 50% | n = 3, 30% |
| 40.6 | ||||
| % GFP MAPC | 32 ± 7 | 49 ± 9 | 74 ± 13 | 30 ± 5 |
. | 1st recipient 1 month after transplantation, n = 4 . | 1st recipient 2 months after transplantation, n = 4 . | 1st recipient 3 months after transplantation, n = 4 . | 2nd recipient 2 months after transplantation, n = 9 . |
|---|---|---|---|---|
| Successful MAPC cx in age-matched mice | 2-mo-old mice 4/6, 66% | 3-mo-old mice 5/11, 45% | 4-mo-old mice 3/7, 42% | 5- to 6-mo-old mice 1/10, 10% |
| Successful MAPC cx in transplant recipients | n = 3, 75% | n = 2, 50% | n = 2, 50% | n = 3, 30% |
| 40.6 | ||||
| % GFP MAPC | 32 ± 7 | 49 ± 9 | 74 ± 13 | 30 ± 5 |
Percentage of successful MAPC cultures, defined as cultures grown for more than 50 cell doublings, capable of differentiating into endothelial cells, hepatocytes, and neurons, in first and second recipient isolations and percentage of GFP-positive cells (determined by FACS analysis) within those populations. Compare to the percentage of successful MAPC cultures in age-matched control mice (nontransplanted, nonirradiated). Cx indicates cultures.