Table 3.

Percent survival of cocultured INA-6 cells on nutlin-3–treated BMSCs

Patient 1
Patient 2
Day 10
Day 22
Day 10
Day 22
DMSO   100   100   100   100  
Nutlin-3a   98   99   100   99  
Nutlin-3b
 		 100
 		 100
 		 100
 		 100
 
Patient 1
Patient 2
Day 10
Day 22
Day 10
Day 22
DMSO   100   100   100   100  
Nutlin-3a   98   99   100   99  
Nutlin-3b
 		 100
 		 100
 		 100
 		 100
 

BMSCs that had been incubated with 10 μM nutlins, or with DMSO, for either 10 days or 22 days, were supplied with fresh medium without drugs and without IL-6, and INA-6 MM cells added. Viability of MM cells was determined after 3 days in coculture by annexin V–FITC/PI FACS analysis. Numbers represent median values from 2 assays. These data show that nutlin-3 treatment has no detrimental effect on the ability of BMSCs to support MM cells and that the antitumor effect of the drug in coculture experiments is therefore likely to be direct.

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