Nutlin effects on clonal growth of CD34+ cells
. | Number of colony-forming units . | . | . | ||
|---|---|---|---|---|---|
| Patient no. . | DMSO . | Nutlin-3a, 8 μM . | Nutlin-3b, 8 μM . | ||
| 1 | 231 | 0 | 230 | ||
| 2 | 280 | 0 | 262 | ||
| 3 | 205 | 0 | 181 | ||
| 4 | 353 | 0 | 403 | ||
| 5 | 298 | 0 | 353 | ||
. | Number of colony-forming units . | . | . | ||
|---|---|---|---|---|---|
| Patient no. . | DMSO . | Nutlin-3a, 8 μM . | Nutlin-3b, 8 μM . | ||
| 1 | 231 | 0 | 230 | ||
| 2 | 280 | 0 | 262 | ||
| 3 | 205 | 0 | 181 | ||
| 4 | 353 | 0 | 403 | ||
| 5 | 298 | 0 | 353 | ||
Treatment of CD34+ cells with 8 μM nutlin-3a precluded any cell growth and differentiation in colony formation assays. The inactive enantiomer was without effect as compared to control incubations with DMSO. CD34+ cells from 5 different donors were tested.