Table 1.

Hematologic parameters of ?/+ and nm/nm animals

4 weeks
8 weeks
12 weeks
24 weeks
Parameter
?/+, n = 8
nm/nm, n = 8
P
?/+, n = 11
nm/nm, n = 10
P
?/+, n = 8
nm/nm, n = 8
P
?/+, n = 10
nm/nm, n = 10
P
WBCs, × 103/μL   2.35 ± 1.40   2.49 ± 0.28   .810   1.30 ± 0.55   4.09 ± 1.65   < .001   4.27 ± 2.63   5.85 ± 1.97   .197   3.32 ± 2.48   5.80 ± 4.18   .127  
RBCs, × 106/μL   8.17 ± 0.83   10.49 ± 1.03   < .001   9.60 ± 0.51   14.01 ± 0.90   < .001   9.30 ± 1.17   15.55 ± 1.04   < .001   9.54 ± 0.52   15.86 ± 0.76   < .001  
Hemoglobin, g/dL   13.1 ± 1.4   6.9 ± 0.5   < .001   15.3 ± 1.0   9.0 ± 0.8   < .001   14.3 ± 1.9   9.1 ± 0.7   < .001   13.9 ± 0.9   8.8 ± 0.7   < .001  
Hematocrit, %   47.3 ± 3.8   33.3 ± 3.4   < .001   50.1 ± 2.0   36.2 ± 2.7   < .001   48.4 ± 5.3   37.0 ± 2.9   < .001   47.3 ± 2.2   37.2 ± 2.2   < .001  
MCV, fL   58.1 ± 2.7   30.4 ± 2.8   < .001   52.3 ± 1.4   25.9 ± 1.3   < .001   52.2 ± 2.6   23.9 ± 2.1   < .001   49.7 ± 2.2   23.5 ± 1.2   < .001  
MCH, pg   16.0 ± 0.7   6.6 ± 0.5   < .001   16.0 ± 0.8   6.4 ± 0.4   < .001   15.4 ± 0.9   5.9 ± 0.4   < .001   14.6 ± 1.2   5.6 ± 0.3   < .001  
MCHC, g/dL   27.7 ± 1.8   20.9 ± 1.5   < .001   30.5 ± 1.3   24.9 ± 1.5   < .001   29.5 ± 0.4   24.6 ± 1.5   < .001   29.4 ± 1.8   23.8 ± 1.1   < .001  
RDW, %   14.4 ± 1.0   31.0 ± 2.2   < .001   13.0 ± 0.8   28.9 ± 1.1   < .001   13.6 ± 0.7   30.1 ± 1.1   < .001   13.5 ± 0.8   28.2 ± 2.6   < .001  
Platelets, × 106/μL   1144 ± 290   3798 ± 958   < .001   1262 ± 159   2550 ± 603   < .001   1154 ± 245   2286 ± 693   .002   1301 ± 129   2183 ± 464   < .001  
MPV, fL   7.8 ± 0.3   10.3 ± 1.7   .004   8.1 ± 0.4   8.2 ± 0.7   .730   8.1 ± 0.4   8.0 ± 0.6   .687   8.3 ± 0.2   8.2 ± 0.8   .785  
Reticulocytes, %   6.1 ± 2.4   21.1 ± 4.4   < .001   1.6 ± 0.4   7.9 ± 2.5   < .001   2.2 ± 0.8   7.3 ± 3.2   .002   2.4 ± 1.3   4.3 ± 1.0   < .001  
Abs retic, × 106/μL   0.491 ± 0.168   2.216 ± 0.518   < .001   0.156 ± 0.041   1.095 ± 0.297   < .001   0.200 ± 0.055   1.126 ± 0.428   < .001   0.222 ± 0.109   0.684 ± 0.146   .002  
CHr, pg   15.1 ± 0.4   7.5 ± 0.2   < .001   14.6 ± 0.5   6.9 ± 0.3   < .001   14.5 ± 0.7   7.1 ± 0.3   < .001   14.6 ± 0.7   7.4 ± 0.3   < .001  
ZnPP/H, μmol/mol
 		 125 ± 47
 		 532 ± 227
 		 .001
 		 74 ± 8
 		 285 ± 66
 		 < .001
 		 74 ± 12
 		 268 ± 65
 		 < .001
 		 75 ± 19
 		 196 ± 39
 		 < .001
 
4 weeks
8 weeks
12 weeks
24 weeks
Parameter
?/+, n = 8
nm/nm, n = 8
P
?/+, n = 11
nm/nm, n = 10
P
?/+, n = 8
nm/nm, n = 8
P
?/+, n = 10
nm/nm, n = 10
P
WBCs, × 103/μL   2.35 ± 1.40   2.49 ± 0.28   .810   1.30 ± 0.55   4.09 ± 1.65   < .001   4.27 ± 2.63   5.85 ± 1.97   .197   3.32 ± 2.48   5.80 ± 4.18   .127  
RBCs, × 106/μL   8.17 ± 0.83   10.49 ± 1.03   < .001   9.60 ± 0.51   14.01 ± 0.90   < .001   9.30 ± 1.17   15.55 ± 1.04   < .001   9.54 ± 0.52   15.86 ± 0.76   < .001  
Hemoglobin, g/dL   13.1 ± 1.4   6.9 ± 0.5   < .001   15.3 ± 1.0   9.0 ± 0.8   < .001   14.3 ± 1.9   9.1 ± 0.7   < .001   13.9 ± 0.9   8.8 ± 0.7   < .001  
Hematocrit, %   47.3 ± 3.8   33.3 ± 3.4   < .001   50.1 ± 2.0   36.2 ± 2.7   < .001   48.4 ± 5.3   37.0 ± 2.9   < .001   47.3 ± 2.2   37.2 ± 2.2   < .001  
MCV, fL   58.1 ± 2.7   30.4 ± 2.8   < .001   52.3 ± 1.4   25.9 ± 1.3   < .001   52.2 ± 2.6   23.9 ± 2.1   < .001   49.7 ± 2.2   23.5 ± 1.2   < .001  
MCH, pg   16.0 ± 0.7   6.6 ± 0.5   < .001   16.0 ± 0.8   6.4 ± 0.4   < .001   15.4 ± 0.9   5.9 ± 0.4   < .001   14.6 ± 1.2   5.6 ± 0.3   < .001  
MCHC, g/dL   27.7 ± 1.8   20.9 ± 1.5   < .001   30.5 ± 1.3   24.9 ± 1.5   < .001   29.5 ± 0.4   24.6 ± 1.5   < .001   29.4 ± 1.8   23.8 ± 1.1   < .001  
RDW, %   14.4 ± 1.0   31.0 ± 2.2   < .001   13.0 ± 0.8   28.9 ± 1.1   < .001   13.6 ± 0.7   30.1 ± 1.1   < .001   13.5 ± 0.8   28.2 ± 2.6   < .001  
Platelets, × 106/μL   1144 ± 290   3798 ± 958   < .001   1262 ± 159   2550 ± 603   < .001   1154 ± 245   2286 ± 693   .002   1301 ± 129   2183 ± 464   < .001  
MPV, fL   7.8 ± 0.3   10.3 ± 1.7   .004   8.1 ± 0.4   8.2 ± 0.7   .730   8.1 ± 0.4   8.0 ± 0.6   .687   8.3 ± 0.2   8.2 ± 0.8   .785  
Reticulocytes, %   6.1 ± 2.4   21.1 ± 4.4   < .001   1.6 ± 0.4   7.9 ± 2.5   < .001   2.2 ± 0.8   7.3 ± 3.2   .002   2.4 ± 1.3   4.3 ± 1.0   < .001  
Abs retic, × 106/μL   0.491 ± 0.168   2.216 ± 0.518   < .001   0.156 ± 0.041   1.095 ± 0.297   < .001   0.200 ± 0.055   1.126 ± 0.428   < .001   0.222 ± 0.109   0.684 ± 0.146   .002  
CHr, pg   15.1 ± 0.4   7.5 ± 0.2   < .001   14.6 ± 0.5   6.9 ± 0.3   < .001   14.5 ± 0.7   7.1 ± 0.3   < .001   14.6 ± 0.7   7.4 ± 0.3   < .001  
ZnPP/H, μmol/mol
 		 125 ± 47
 		 532 ± 227
 		 .001
 		 74 ± 8
 		 285 ± 66
 		 < .001
 		 74 ± 12
 		 268 ± 65
 		 < .001
 		 75 ± 19
 		 196 ± 39
 		 < .001
 

Complete blood counts and red blood cell zinc ratios of protoporphyrin to heme (ZnPP/H) were determined in 129B6F2 ?/+ and nm/nm animals at 4, 8, 12, and 24 weeks of age. Values are presented as the mean ± 1 SD. Statistical significance values (P) are reported for the two-tailed Student t test. WBCs indicates white blood cells; MCV, mean cell volume; MCH, mean cell hemoglobin; MCHC, mean cell hemoglobin concentration; RDW, red cell distribution width; MPV, mean platelet volume; Abs retic, absolute reticulocytes; CHr, mean cellular hemoglobin of the reticulocytes.

Conversion to SI units is as follows: WBCs, Abs retic, and platelets, × 106 to achieve units in × 109/L; RBCs, × 106 to achieve units expressed in × 1012/L; hemoglobin and hematocrit, × 10 to achieve units in g/L; serum iron and TIBC, × 0.179 to achieve units in μM.

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