Cytotoxic activity of primary CTLs from PBSC harvests in MM patients after stimulation with HM1.24 peptide–pulsed DCs
. | HLA-A2+, A24+ . | . | HLA-A2+, A24- . | . | HLA-A2-, A24+ . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| Peptide . | Patient 1 . | Patient 2 . | Patient 3 . | Patient 4 . | Patient 5 . | Patient 6 . | |||
| HM1.24-126 | ++ | + | ++ | ++ | – | + | |||
| HM1.24-136 | – | – | – | – | – | – | |||
| HM1.24-152 | + | – | – | – | – | – | |||
| HM1.24-165 | ++ | – | – | + | ++ | – | |||
| MAGE-3-195 | – | – | – | – | + | – | |||
. | HLA-A2+, A24+ . | . | HLA-A2+, A24- . | . | HLA-A2-, A24+ . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| Peptide . | Patient 1 . | Patient 2 . | Patient 3 . | Patient 4 . | Patient 5 . | Patient 6 . | |||
| HM1.24-126 | ++ | + | ++ | ++ | – | + | |||
| HM1.24-136 | – | – | – | – | – | – | |||
| HM1.24-152 | + | – | – | – | – | – | |||
| HM1.24-165 | ++ | – | – | + | ++ | – | |||
| MAGE-3-195 | – | – | – | – | + | – | |||
Nonattached fractions of PBSC harvests were stimulated with peptide-pulsed DCs generated also from PBSC harvests. Five days after the second stimulation, CD8+ T cells were isolated using MACS beads and further cultured for 4 days. Cytotoxic activity of these CTLs was tested against YN cells that had been pulsed with relevant peptides. Cytotoxicity was determined by granzyme B ELISPOT assay and CTL responses were evaluated by the number of spots (–, < 15 spots; +, 15-30 spots; and ++, 31-60 spots).