Rabbit groups and inocula
Rabbit . | Inoculum type* . | No. of cells, × 107 . |
|---|---|---|
| R1 | ACH.95 | 1 |
| R2 | ACH.95 | 1 |
| R16 | ACH.95 | 1.43 |
| R17 | ACH.95 | 1.43 |
| R11 | ACH.75 | 1 |
| R12 | ACH.75 | 1 |
| R13 | ACH.75 | 1 |
| R14 | ACH.75 | 1 |
| R18 | ACH.195 | 1.35 |
| R19 | ACH.195 | 1.35 |
| R20 | ACH.195 | 1.35 |
| R21 | ACH.195 | 1.35 |
| R5 | ACH.1 | 1 |
| R10 | ACH.1 | 1 |
| R22 | ACH.1 | 1 |
| R9 | PBMC | 1 |
Rabbit . | Inoculum type* . | No. of cells, × 107 . |
|---|---|---|
| R1 | ACH.95 | 1 |
| R2 | ACH.95 | 1 |
| R16 | ACH.95 | 1.43 |
| R17 | ACH.95 | 1.43 |
| R11 | ACH.75 | 1 |
| R12 | ACH.75 | 1 |
| R13 | ACH.75 | 1 |
| R14 | ACH.75 | 1 |
| R18 | ACH.195 | 1.35 |
| R19 | ACH.195 | 1.35 |
| R20 | ACH.195 | 1.35 |
| R21 | ACH.195 | 1.35 |
| R5 | ACH.1 | 1 |
| R10 | ACH.1 | 1 |
| R22 | ACH.1 | 1 |
| R9 | PBMC | 1 |
Twelve-week-old specific pathogen-free New Zealand white rabbits were inoculated via the lateral ear vein as described in “Materials and methods.” ACH.95, ACH.75, ACH.195, and ACH.1 cell lines were obtained by outgrowth of immortalized PBMCs previously transfected with the full-length HTLV-1 molecular clones ACH.95, ACH.75, ACH.195, and ACH, respectively.30,31 Inocula were equilibrated by p19 gag production on a per-cell basis prior to inoculation