Distribution of c-kit+ Thy-1.1lo Lin−/lo Sca-1+ Cells in the Different Tissues After G-CSF Mobilization
| . | Tissue source . | No. Cells/Tissue* . | % KTLS . | No. KTLS/Tissue . |
|---|---|---|---|---|
| Experiment I | Control PB/mL | 4.2 × 106 | <0.01 | — |
| Control BM | 4.8 × 107 | 0.07 | 3.4 × 104 | |
| Control SPL | 1.5 × 108 | <0.01 | — | |
| G-CSF PB/mL | 3.5 × 107 | 0.04 | 1.5 × 104/mL | |
| G-CSF BM | 3.7 × 107 | 0.04 | 1.6 × 104 | |
| G-CSF SPL | 2.3 × 108 | 0.25 | 5.8 × 105 | |
| Experiment II | Control PB/mL | 8.6 × 106 | <0.01 | — |
| Control BM | 3.6 × 107 | 0.08 | 3.0 × 104 | |
| Control SPL | 8.4 × 107 | <0.01 | — | |
| G-CSF PB/mL | 2.1 × 107 | 0.02 | 5.0 × 103/mL | |
| G-CSF BM | 3.4 × 107 | 0.07 | 2.4 × 104 | |
| G-CSF SPL | 1.1 × 108 | 0.37 | 4.2 × 105 | |
| Experiment III | Control PB/mL | 6.4 × 106 | <0.01 | — |
| Control BM | 3.6 × 107 | 0.08 | 3.0 × 104 | |
| Control SPL | 3.1 × 107 | <0.01 | — | |
| Splenectomized | G-CSF PB/mL | 7.2 × 107 | 0.01 | 9.3 × 103/mL |
| Splenectomized | G-CSF BM | 3.7 × 107 | 0.07 | 2.7 × 104 |
| . | Tissue source . | No. Cells/Tissue* . | % KTLS . | No. KTLS/Tissue . |
|---|---|---|---|---|
| Experiment I | Control PB/mL | 4.2 × 106 | <0.01 | — |
| Control BM | 4.8 × 107 | 0.07 | 3.4 × 104 | |
| Control SPL | 1.5 × 108 | <0.01 | — | |
| G-CSF PB/mL | 3.5 × 107 | 0.04 | 1.5 × 104/mL | |
| G-CSF BM | 3.7 × 107 | 0.04 | 1.6 × 104 | |
| G-CSF SPL | 2.3 × 108 | 0.25 | 5.8 × 105 | |
| Experiment II | Control PB/mL | 8.6 × 106 | <0.01 | — |
| Control BM | 3.6 × 107 | 0.08 | 3.0 × 104 | |
| Control SPL | 8.4 × 107 | <0.01 | — | |
| G-CSF PB/mL | 2.1 × 107 | 0.02 | 5.0 × 103/mL | |
| G-CSF BM | 3.4 × 107 | 0.07 | 2.4 × 104 | |
| G-CSF SPL | 1.1 × 108 | 0.37 | 4.2 × 105 | |
| Experiment III | Control PB/mL | 6.4 × 106 | <0.01 | — |
| Control BM | 3.6 × 107 | 0.08 | 3.0 × 104 | |
| Control SPL | 3.1 × 107 | <0.01 | — | |
| Splenectomized | G-CSF PB/mL | 7.2 × 107 | 0.01 | 9.3 × 103/mL |
| Splenectomized | G-CSF BM | 3.7 × 107 | 0.07 | 2.7 × 104 |
Mice were treated with 250 μg/kg/d of G-CSF or PBS/BSA as a control for 7 days. Cells obtained from PB, BM from four long bones representing 15% to 20% of total BM, and spleen (SPL) were purified according to the phenotypic markers c-kit+ Thy-1.1lo Lin−/lo Sca-1+ and the percentage of KTLS cells isolated from each tissue is shown.
Numbers of cells per milliliter of PB.