Cell Cycle Status of Human CD34+ Thy-1+ Lin− Cells
| Tissue . | Population . | % S/G2/M . | n . |
|---|---|---|---|
| BM | CD34+ Thy-1+ Lin− | 6.4 ± 1.4 | 5 |
| CD34+ Thy-1− Lin− | 6.3 ± 1.3 | 5 | |
| MPB (G-CSF) | CD34+ Thy-1+ Lin− | <0.2 | 5 |
| CD34+ Thy-1− Lin− | <0.5 | 5 | |
| MPB (CTX/GM) | CD34+ Thy-1+ Lin− | <0.1 | 2 |
| CD34+ Thy-1+ | 0.33 | 1 | |
| CD34+ Thy-1− | 0.7 | 1 |
| Tissue . | Population . | % S/G2/M . | n . |
|---|---|---|---|
| BM | CD34+ Thy-1+ Lin− | 6.4 ± 1.4 | 5 |
| CD34+ Thy-1− Lin− | 6.3 ± 1.3 | 5 | |
| MPB (G-CSF) | CD34+ Thy-1+ Lin− | <0.2 | 5 |
| CD34+ Thy-1− Lin− | <0.5 | 5 | |
| MPB (CTX/GM) | CD34+ Thy-1+ Lin− | <0.1 | 2 |
| CD34+ Thy-1+ | 0.33 | 1 | |
| CD34+ Thy-1− | 0.7 | 1 |
The defined CD34+ populations were obtained from these sources: BM of untreated healthy donor; G-CSF–mobilized MPB from a healthy donor; and CTX/GM-CSF–mobilized MPB from cancer patients. CD34+ Thy-1+ Lin− and CD34+ Thy-1− Lin− cells from these sample were sorted, stained with the Hoechst 33342, and analyzed for their DNA content. One sample from CTX/GM MPB, the CD34+ enriched population, was stained with Hoechst 33342 and MoAbs against CD34 and Thy-1 as shown in Fig 4c. The percentage of CD34+ Thy-1+ Lin− cells in S/G2/M from MPB was significantly less than that from BM (P < .0005).