BFUe In Vitro Differentiation Assay
| Factor . | Experiment 1 (19 d) . | Experiment 2 (20 d) . | ||
|---|---|---|---|---|
| . | Concentration (nmol/L) . | No. of Colonies (duplicates) . | Concentration (nmol/L) . | No. of Colonies (duplicates) . |
| EPO | 0.0013 | 9/11 | ||
| 0.0026 | 30/22 | 0.0026 | 31/27 | |
| 0.0065 | 31/37 | 0.0065 | 46/62 | |
| 0.0130 | 49/56 | 0.0130 | 81/97 | |
| 0.0259 | 55/61 | 0.0259 | 142/100 | |
| 0.0649 | 73/63 | |||
| 0.130 | 133/126 | |||
| MoAb34 | 1.8 | 0/0 | ||
| 3.6 | 0/0 | 3.6 | 0/0 | |
| 7.2 | 0/2 | 7.2 | 0/0 | |
| 14.4 | 5/6 | |||
| 21.6 | 12/10 | 21.6 | 10/7 | |
| 36.0 | 15/3 | 36.0 | 13/12 | |
| 72.0 | 10/7 | 72.0 | 7/15 | |
| 144 | 5/14 | |||
| 216 | 11/4 | 216 | 4/13 | |
| 360 | 4/3 | 360 | 6/9 | |
| 720 | 2/6 | 720 | 0/2 | |
| 1,080 | 0/1 | 1,080 | 0/0 | |
| 1,800 | 0/1 | |||
| MoAb control | 6.9 | 0/0 | ||
| 34.4 | 0/0 | 34.4 | 0/0 | |
| 206 | 0/0 | 206 | 0/0 | |
| 344 | 0/0 | |||
| 688 | 0/0 | |||
| Factor . | Experiment 1 (19 d) . | Experiment 2 (20 d) . | ||
|---|---|---|---|---|
| . | Concentration (nmol/L) . | No. of Colonies (duplicates) . | Concentration (nmol/L) . | No. of Colonies (duplicates) . |
| EPO | 0.0013 | 9/11 | ||
| 0.0026 | 30/22 | 0.0026 | 31/27 | |
| 0.0065 | 31/37 | 0.0065 | 46/62 | |
| 0.0130 | 49/56 | 0.0130 | 81/97 | |
| 0.0259 | 55/61 | 0.0259 | 142/100 | |
| 0.0649 | 73/63 | |||
| 0.130 | 133/126 | |||
| MoAb34 | 1.8 | 0/0 | ||
| 3.6 | 0/0 | 3.6 | 0/0 | |
| 7.2 | 0/2 | 7.2 | 0/0 | |
| 14.4 | 5/6 | |||
| 21.6 | 12/10 | 21.6 | 10/7 | |
| 36.0 | 15/3 | 36.0 | 13/12 | |
| 72.0 | 10/7 | 72.0 | 7/15 | |
| 144 | 5/14 | |||
| 216 | 11/4 | 216 | 4/13 | |
| 360 | 4/3 | 360 | 6/9 | |
| 720 | 2/6 | 720 | 0/2 | |
| 1,080 | 0/1 | 1,080 | 0/0 | |
| 1,800 | 0/1 | |||
| MoAb control | 6.9 | 0/0 | ||
| 34.4 | 0/0 | 34.4 | 0/0 | |
| 206 | 0/0 | 206 | 0/0 | |
| 344 | 0/0 | |||
| 688 | 0/0 | |||
Purified CD34+ cells (10,000 cells per duplicate sample) were incubated in methylcellulose in the presence of the indicated sample at different concentrations. Erythroid colonies were counted after 19 days (experiment 1) or 20 days (experiment 2), respectively. For each experiment, the number of colonies of both duplicates are given.