FISH Analysis of Artificial Mixtures of Male (M) and Female (F) PB Mononuclear Cells
| Composition of Samples (%) . | Experiment 1 (%) . | Experiment 2 (%) . | |||
|---|---|---|---|---|---|
| M . | F . | M . | F . | M . | F . |
| 0 | 100 | 0 | 88 | 0 | 90 |
| 0.05 | 99.95 | 0 | 88 | 0 | 90 |
| 0.10 | 99.90 | 0 | 88 | 2 | 91 |
| 0.20 | 99.80 | 0 | 89 | 1 | 91 |
| 1.0 | 99.0 | 1 | 89 | 2 | 93 |
| 2.0 | 98.0 | 2 | 90 | 2 | 94 |
| 5.0 | 95.0 | 4 | 86 | 6 | 89 |
| 100 | 0 | 91 | 0 | 90 | 0 |
| Composition of Samples (%) . | Experiment 1 (%) . | Experiment 2 (%) . | |||
|---|---|---|---|---|---|
| M . | F . | M . | F . | M . | F . |
| 0 | 100 | 0 | 88 | 0 | 90 |
| 0.05 | 99.95 | 0 | 88 | 0 | 90 |
| 0.10 | 99.90 | 0 | 88 | 2 | 91 |
| 0.20 | 99.80 | 0 | 89 | 1 | 91 |
| 1.0 | 99.0 | 1 | 89 | 2 | 93 |
| 2.0 | 98.0 | 2 | 90 | 2 | 94 |
| 5.0 | 95.0 | 4 | 86 | 6 | 89 |
| 100 | 0 | 91 | 0 | 90 | 0 |
FISH data are given for two independent experiments. One thousand interphase nuclei were enumerated for each titration mixture. Columns 1 and 2 refer to the percentages of male and female cells in each sample as calculated from nucleated cell counts. Columns 3-6 pertain to FISH analysis, using directly conjugated X BODIPY-TR and Y BODIPY-FL-X DNA probes, of the percentages of XY (male: M) or XX (female: F) hybridization signals in each test cell mixture.