Clonal Integration Analysis of bnx/hu Bone Marrow by Inverse PCR
Mouse No. . | Transduction Conditions . | Total No. of Proviral Integrants . | Average No. of Integrants/Recipient . |
---|---|---|---|
43A1 | SNS | 0 | |
43A2 | SNS | 0 | |
44A1 | SNS | 0 | |
44A2 | SNS | 0 | |
44A3 | SNS | 0 | |
48A1 | SNS | 0 | |
48A2 | SNS | 0 | |
48A3 | SNS | 0 | 0 |
43B1 | SNS + FL | 1 | |
43B2 | SNS + FL | 2 | |
44B1 | SNS + FL | 0 | |
44B2 | SNS + FL | 0 | |
44B3 | SNS + FL | 1 | |
48B1 | SNS + FL | 2 | |
48B2 | SNS + FL | 2 | |
48B3 | SNS + FL | 0 | 0.88 |
43C1 | SOS | 3 | |
43C2 | SOS | 2 | |
43C3 | SOS | 1 | |
44C1 | SOS | 1 | |
44C2 | SOS | 4 | |
48C1 | SOS | 4 | |
48C2 | SOS | 0 | |
48C3 | SOS | 6 | 2.63 |
Mouse No. . | Transduction Conditions . | Total No. of Proviral Integrants . | Average No. of Integrants/Recipient . |
---|---|---|---|
43A1 | SNS | 0 | |
43A2 | SNS | 0 | |
44A1 | SNS | 0 | |
44A2 | SNS | 0 | |
44A3 | SNS | 0 | |
48A1 | SNS | 0 | |
48A2 | SNS | 0 | |
48A3 | SNS | 0 | 0 |
43B1 | SNS + FL | 1 | |
43B2 | SNS + FL | 2 | |
44B1 | SNS + FL | 0 | |
44B2 | SNS + FL | 0 | |
44B3 | SNS + FL | 1 | |
48B1 | SNS + FL | 2 | |
48B2 | SNS + FL | 2 | |
48B3 | SNS + FL | 0 | 0.88 |
43C1 | SOS | 3 | |
43C2 | SOS | 2 | |
43C3 | SOS | 1 | |
44C1 | SOS | 1 | |
44C2 | SOS | 4 | |
48C1 | SOS | 4 | |
48C2 | SOS | 0 | |
48C3 | SOS | 6 | 2.63 |
Genomic DNA samples extracted from the marrow of each mouse were subjected to clonal analysis by inverse PCR. The number of proviral integrants detected in each sample (no. of PCR product bands/2 LTR bands per proviral integrant = no. of integrants) is listed.