In Vitro Release of p15 and BPI Induced by Calcium and Ionomycin Results in Extracellular Molar Ratios Similar to Those Seen In Vivo
| Calcium (μmol/L) . | p15* . | BPI . | p15/BPI (mol/mol) . |
|---|---|---|---|
| 0 | 0 | ND | — |
| 25† | <4 | ND | — |
| 100‡ | 8 ± 4 | ND | — |
| 250 | 22 ± 7 | 3 ± 2 | 72 |
| 500 | 26 ± 7 | 9 ± 6 | 42 |
| 1,000 | 30 ± 6 | 17 ± 11 | 31 |
| Calcium (μmol/L) . | p15* . | BPI . | p15/BPI (mol/mol) . |
|---|---|---|---|
| 0 | 0 | ND | — |
| 25† | <4 | ND | — |
| 100‡ | 8 ± 4 | ND | — |
| 250 | 22 ± 7 | 3 ± 2 | 72 |
| 500 | 26 ± 7 | 9 ± 6 | 42 |
| 1,000 | 30 ± 6 | 17 ± 11 | 31 |
Differential release of primary and secondary granules by PMNs was induced by ionomycin and extracellular calcium doses as described in Materials and Methods. The percent release of total cellular content is presented as the mean ± SEM of three separate experiments.
Abbreviation: ND, none detected.
Percent released of total cellular content, mean ± SEM (n = 3).
Corresponds to pooled data from 12.5-μmol/L and 32.5-μmol/L calcium doses.
Corresponds to pooled data from 63-μmol/L and 125-μmol/L calcium doses.