Sequences and Locations of Oligonucleotide Primers Used in the PCR Amplification
| Protein . | Primers . | Sense . | Antisense . | Location . |
|---|---|---|---|---|
| GPIbα | Ib1 | AGGTCTTTCTGCCTGCCTGT | TAGCCAGACTGAGCTTCTCC | 280-1053 |
| Ib2 | AAGGCAATGAGCTGAAGACC | CTTGTGTTGGATGCAAGGAG | 979-1575 | |
| Ib3 | TCCACTGCTTCTCTAGACAG | GGCTGATCAAGTTCAGGGAT | 1524-1960 | |
| Ib4 | CACAAGCCTGATCACTCCAA | TTCTCTCAAGGTCCCCAAAC | 1862-2496 | |
| GPIbβ | Ibb | TTACTGCGGCGCTTCCCTTG | GAGTTTGCAGGCCCGTGTTG | 641-1335 |
| Ibb3 | GCAACAACCTGACGGCGCTG | AAGGCCCAGCAGCGCAAGCT | 844-1133 | |
| GPIX | IX | TGTTCCTGCTCTGGGCCACA | TTGGTGGAGTCTGGGGACCT | 981-1560 |
| vWF | vW24 | CTGGATGTGGCGTTCGTCCT | CTCAAAGTCCTGGATGAGGATA | 4489-4965 |
| Protein . | Primers . | Sense . | Antisense . | Location . |
|---|---|---|---|---|
| GPIbα | Ib1 | AGGTCTTTCTGCCTGCCTGT | TAGCCAGACTGAGCTTCTCC | 280-1053 |
| Ib2 | AAGGCAATGAGCTGAAGACC | CTTGTGTTGGATGCAAGGAG | 979-1575 | |
| Ib3 | TCCACTGCTTCTCTAGACAG | GGCTGATCAAGTTCAGGGAT | 1524-1960 | |
| Ib4 | CACAAGCCTGATCACTCCAA | TTCTCTCAAGGTCCCCAAAC | 1862-2496 | |
| GPIbβ | Ibb | TTACTGCGGCGCTTCCCTTG | GAGTTTGCAGGCCCGTGTTG | 641-1335 |
| Ibb3 | GCAACAACCTGACGGCGCTG | AAGGCCCAGCAGCGCAAGCT | 844-1133 | |
| GPIX | IX | TGTTCCTGCTCTGGGCCACA | TTGGTGGAGTCTGGGGACCT | 981-1560 |
| vWF | vW24 | CTGGATGTGGCGTTCGTCCT | CTCAAAGTCCTGGATGAGGATA | 4489-4965 |
The oligonucleotide primers for the GPIbα, GPIbβ, and GPIX genes are numbered according to Wenger et al,13 Yagi et al,14 and Hickey and Roth,15 respectively. The nucleotide positions within the vWF gene are based on the sequence of Bonthron et al.53