The Presence of Ph+CD34+CD38− CML Cells Has No Effect on the Expansion of Normal LTC-IC in 10-Day Cocultures
| Source of CML Cells Added (sex) . | No. of Day 10 LTC-IC . | No. of Day 10 LTC-IC per 100 Input CML Cells Cultured in the Presence of 100 Normal BM Cells . | % Ph− Day 10 LTC-IC . | Expansion of Normal LTC-IC6-150 . |
|---|---|---|---|---|
| . | per 100 Input CML Cells in the Absence of Normal BM Cells (fold change) . | . | (no. Ph−/total analyzed, sex) . | . |
| CML no. 1 (M) | <0.025 | 263 | 100 (21/21, F) | 10 x |
| CML no. 2 (F) | <0.025 | 1,125 | 100 (27/27, F) | 42 x |
| CML no. 5 (M) | <0.025 | 1,225 | 97 (33/34, F)6-151 | 45 x |
| None | 450 | — | 17 x |
| Source of CML Cells Added (sex) . | No. of Day 10 LTC-IC . | No. of Day 10 LTC-IC per 100 Input CML Cells Cultured in the Presence of 100 Normal BM Cells . | % Ph− Day 10 LTC-IC . | Expansion of Normal LTC-IC6-150 . |
|---|---|---|---|---|
| . | per 100 Input CML Cells in the Absence of Normal BM Cells (fold change) . | . | (no. Ph−/total analyzed, sex) . | . |
| CML no. 1 (M) | <0.025 | 263 | 100 (21/21, F) | 10 x |
| CML no. 2 (F) | <0.025 | 1,125 | 100 (27/27, F) | 42 x |
| CML no. 5 (M) | <0.025 | 1,225 | 97 (33/34, F)6-151 | 45 x |
| None | 450 | — | 17 x |
All cultures contained CD34+CD38− cells isolated from a normal female (F) bone marrow (BM) sample and 100 ng/mL of FL and SF, and 20 ng/mL of IL-3, IL-6, and G-CSF. CD34+CD38− CML cells were added as shown.
Abbreviation: ND, not done.
Day 10 LTC-IC value relative to number of LTC-IC in 100 input normal BM cells (=27).
In the cocultures of normal female marrow plus CML no. 3 cells, 1 Ph+ (male) colony was found in the LTC-IC assays.