Effect of Irradiation Dose on the Level of Chimerism and Skin Graft Survival in B10 Hosts Treated With Haploidentical Bone Marrow Cells of (B10 × B10.D2) F1 Mice
| Group . | Animal No. . | TBI . | % Donor-Type Cells† . | Skin Graft Survival‡ (d) . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | (Gy)* . | Lymph Nodes . | Blood . | Donor Tx . | Third-Party Tx . | . | . | ||
| . | . | . | (day 49) . | (day 119) . | First . | Secondρ . | First . | Secondρ . | . | . |
| 1 | 11 | 3.0 | 68.7 | 49.5 | >120 | >120 | 21 | 22 | ||
| 12 | 28.4 | 49.1 | >120 | >120 | 70 | 32 | ||||
| 13 | 59.1 | 54.4 | >120 | >120 | 60 | 22 | ||||
| 14 | 57.5 | 59.1 | >120 | >120 | 46 | 22 | ||||
| 15 | 68.7 | 66.4 | >120 | >120 | 64 | 32 | ||||
| 16 | 65.7 | 61.8 | >120 | >120 | 60 | 22 | ||||
| 2 | 17 | 6.0 | 89.7 | 87.1 | >120 | >120 | 70 | 22 | ||
| 18 | 90.3 | 90.7 | >120 | >120 | 60 | 32 | ||||
| 19 | 73.0 | 86.7 | >120 | >120 | 75 | 25 | ||||
| 20 | 86.8 | 92.8 | >120 | >120 | 75 | 22 | ||||
| Control host, immunosuppressed1-155 | 0.4 | 0.2 | 62 | 22 | 75 | 32 | ||||
| Control host, untreated | 0.6 | 0.6 | 25 | 21 | 25 | 22 | ||||
| Control donor | 98.6 | 99.3 | ||||||||
| Group . | Animal No. . | TBI . | % Donor-Type Cells† . | Skin Graft Survival‡ (d) . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | (Gy)* . | Lymph Nodes . | Blood . | Donor Tx . | Third-Party Tx . | . | . | ||
| . | . | . | (day 49) . | (day 119) . | First . | Secondρ . | First . | Secondρ . | . | . |
| 1 | 11 | 3.0 | 68.7 | 49.5 | >120 | >120 | 21 | 22 | ||
| 12 | 28.4 | 49.1 | >120 | >120 | 70 | 32 | ||||
| 13 | 59.1 | 54.4 | >120 | >120 | 60 | 22 | ||||
| 14 | 57.5 | 59.1 | >120 | >120 | 46 | 22 | ||||
| 15 | 68.7 | 66.4 | >120 | >120 | 64 | 32 | ||||
| 16 | 65.7 | 61.8 | >120 | >120 | 60 | 22 | ||||
| 2 | 17 | 6.0 | 89.7 | 87.1 | >120 | >120 | 70 | 22 | ||
| 18 | 90.3 | 90.7 | >120 | >120 | 60 | 32 | ||||
| 19 | 73.0 | 86.7 | >120 | >120 | 75 | 25 | ||||
| 20 | 86.8 | 92.8 | >120 | >120 | 75 | 22 | ||||
| Control host, immunosuppressed1-155 | 0.4 | 0.2 | 62 | 22 | 75 | 32 | ||||
| Control host, untreated | 0.6 | 0.6 | 25 | 21 | 25 | 22 | ||||
| Control donor | 98.6 | 99.3 | ||||||||
Abbreviation: Tx, transplantation.
B10 host mice received anti-CD4, anti-CD3, 15.106 F1 donor bone marrow cells, 3 or 6 Gy TBI and skin grafts of host, donor, and third party (B10.BR) type (day 0).
% donor-type cells (H-2b pos/d pos) + % host-type cells (H-2b pos/d neg) was always 95% to 100%.
Syngeneic skin graft survival was 100%.
ρ Second skin transplantation was performed on day 120.
Control mice, treated with anti-CD4, anti-CD3, and 6 Gy TBI.