Cytotoxicity of Recombinant Toxins Containing Human GM-CSF
| Cell Line . | Cell Type . | IC50 (ng/mL) ± SD . | |
|---|---|---|---|
| . | . | GM-CSF–PE38KDEL . | DT388–GM-CSF . |
| LS174T | Colon | 2.2 ± 0.7 | 70 ± 18 |
| SW403 | Colon | 0.9 ± 0.5 | 15 ± 0.6 |
| N87 | Gastric | 0.45 ± 0.2 | 3.7 ± 0.1 |
| HTB-103 | Gastric | 9.5 ± 7.5 | 400 ± 300 |
| HL60 | Promyelocytic | > 100 | 0.4 ± 0.2 |
| TF-1 | Erythroleukemia | 22 ± 8 | 0.02 ± 0.01 |
| U937 | Monocytic | 9.5 ± 5.5 | 0.04 ± 0.02 |
| Cell Line . | Cell Type . | IC50 (ng/mL) ± SD . | |
|---|---|---|---|
| . | . | GM-CSF–PE38KDEL . | DT388–GM-CSF . |
| LS174T | Colon | 2.2 ± 0.7 | 70 ± 18 |
| SW403 | Colon | 0.9 ± 0.5 | 15 ± 0.6 |
| N87 | Gastric | 0.45 ± 0.2 | 3.7 ± 0.1 |
| HTB-103 | Gastric | 9.5 ± 7.5 | 400 ± 300 |
| HL60 | Promyelocytic | > 100 | 0.4 ± 0.2 |
| TF-1 | Erythroleukemia | 22 ± 8 | 0.02 ± 0.01 |
| U937 | Monocytic | 9.5 ± 5.5 | 0.04 ± 0.02 |
Cells were plated in 96-well plates at 1.5 × 104/well and incubated at 37°C for 24 hours. The cells were then incubated with recombinant toxins for 24 to 48 hours and [3H]-leucine for 4 to 6 hours.