Engraftment of Male Donor Cells Into Female Hosts
| Percent Male Chimerism in Bone Marrow . | |||
|---|---|---|---|
| Animal . | Total Cells Injected . | Y Chromosome FISH . | Southern Blot PY2 . |
| TX1 | 40 × 106 | 8.9 | 9.1 |
| TX2 | 60 × 106 | 9.8 | 7.0 |
| TX5 | 44 × 106 | 9.7 | 6.5 |
| TX6 | 35 × 106 | 13.4 | 7.1 |
| Percent Male Chimerism in Bone Marrow . | |||
|---|---|---|---|
| Animal . | Total Cells Injected . | Y Chromosome FISH . | Southern Blot PY2 . |
| TX1 | 40 × 106 | 8.9 | 9.1 |
| TX2 | 60 × 106 | 9.8 | 7.0 |
| TX5 | 44 × 106 | 9.7 | 6.5 |
| TX6 | 35 × 106 | 13.4 | 7.1 |
Bone marrow from each transplant recipient was assayed for the percent of donor cell composition. The experiment label for each animal appears in the first column (TX1, TX2, TX5, TX6). The total number of male bone marrow cells injected into each female is listed in the second column. The donor engraftment value determined by counting individual cells from bone marrow smears are listed in column three. A positive signal in the fluorescence in situ hybridization (FISH) assay is produced with a Y-chromosome–specific painting probe. To determine the percent male, the number of cells positive by FISH was divided by the total number of cells in each sample. Total cell counts were obtained by staining nuclear chromatin of all cells with 4,6-Diamidino-2-phenylindole (DAPI). Several entire fields were counted for each group (136 to 480 cells/group) by three individuals and the results were averaged. Bone marrow DNA from each mouse was analyzed by Southern blot using the PY2 plasmid.29 The resulting donor engraftment values for whole bone marrow DNA are presented in column four.