Engagement of HLA Class I by MoAb90 and YTH862 Induces Apoptosis of CD40 Ligand-Activated B Cells
| . | CD40 Ligand* . | CD40 Ligand-CD32* . | ||
|---|---|---|---|---|
| . | Apoptotic Cells . | Viable Cells . | Apoptotic Cells . | Viable Cells . |
| . | (%) . | (%) . | (%) . | (%) . |
| Medium | 25.2 | 63.2 | 36.0 | 57.2 |
| Control IgG1 | 20.5 | 62.0 | 35.3 | 60.5 |
| MoAb90 | 49.1 | 12.4 | 57.7 | 21.2 |
| YTH862 | 45.3 | 37.0 | 51.0 | 38.0 |
| W6/32 | 23.3 | 63.6 | 30.7 | 59.4 |
| B9.12.1 | 28.2 | 63.7 | 39.4 | 59.8 |
| TP25.99 | 28.5 | 59.7 | 36.6 | 59.9 |
| . | CD40 Ligand* . | CD40 Ligand-CD32* . | ||
|---|---|---|---|---|
| . | Apoptotic Cells . | Viable Cells . | Apoptotic Cells . | Viable Cells . |
| . | (%) . | (%) . | (%) . | (%) . |
| Medium | 25.2 | 63.2 | 36.0 | 57.2 |
| Control IgG1 | 20.5 | 62.0 | 35.3 | 60.5 |
| MoAb90 | 49.1 | 12.4 | 57.7 | 21.2 |
| YTH862 | 45.3 | 37.0 | 51.0 | 38.0 |
| W6/32 | 23.3 | 63.6 | 30.7 | 59.4 |
| B9.12.1 | 28.2 | 63.7 | 39.4 | 59.8 |
| TP25.99 | 28.5 | 59.7 | 36.6 | 59.9 |
Total B cells were activated for 5 days with CD40-L–transfected L cells or with CD40-L/CD32-double transfected L cells as described in Materials and Methods. Percentages of apoptotic cells and viable cells were determined by TUNEL and propidium iodide incorporation, respectively, after 4 hours of treatment with the different antibodies.