Two-Color and One-Color Flow Cytometric Analysis of CD59/MACIF and/or DAF Expression by Erythroblasts
| . | . | . | . | One-Color Analysis (%) . | |||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | . | . | . |
| . | Two-Color Analysis (%) . | CD59/MACIF . | DAF . | ||||||
| . | . | . | . | . | . | . | . | . | . |
| Case No. . | Positive . | Intermediate . | Negative . | Positive . | Intermediate . | Negative . | Positive . | Intermediate . | Negative . |
| PNH patients | |||||||||
| 1 | 59.5 | 4.9 | 35.6 | 55.9 | 5.7 | 38.4 | 19.3 | 44.7 | 35.9 |
| 2 | 12.6 | 6.7 | 80.8 | 11.3 | 4.8 | 83.8 | 4.3 | 8.7 | 87.0 |
| 3 | 60.0 | 23.7 | 16.3 | 59.9 | 21.5 | 18.6 | 55.1 | 27.5 | 17.4 |
| 4 | 44.1 | 16.9 | 39.0 | 37.0 | 18.0 | 45.0 | 47.3 | 36.1 | 16.6 |
| 5 | 37.6 | 12.6 | 49.8 | 36.4 | 19.7 | 43.9 | 16.8 | 34.7 | 48.5 |
| 6 | 15.1 | 49.1 | 35.8 | 10.7 | 42.4 | 46.9 | 10.6 | 42.9 | 46.5 |
| 7 | — | 62.4 | 37.6 | — | 48.0 | 52.0 | — | 52.1 | 47.9 |
| 8 | 16.4 | 30.0 | 53.6 | 8.4 | 24.9 | 66.7 | 14.7 | 32.5 | 52.8 |
| 9 | — | 19.5 | 80.4 | — | 8.5 | 91.5 | — | 13.2 | 86.8 |
| Normal individuals | |||||||||
| 1 | 98.9 | — | — | 99.0 | — | — | 98.4 | — | — |
| 2 | 98.7 | — | — | 98.9 | — | — | 98.4 | — | — |
| 3 | 98.1 | — | — | 98.3 | — | — | 97.5 | — | — |
| 4 | 99.0 | — | — | 99.1 | — | — | 98.9 | — | — |
| . | . | . | . | One-Color Analysis (%) . | |||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | . | . | . |
| . | Two-Color Analysis (%) . | CD59/MACIF . | DAF . | ||||||
| . | . | . | . | . | . | . | . | . | . |
| Case No. . | Positive . | Intermediate . | Negative . | Positive . | Intermediate . | Negative . | Positive . | Intermediate . | Negative . |
| PNH patients | |||||||||
| 1 | 59.5 | 4.9 | 35.6 | 55.9 | 5.7 | 38.4 | 19.3 | 44.7 | 35.9 |
| 2 | 12.6 | 6.7 | 80.8 | 11.3 | 4.8 | 83.8 | 4.3 | 8.7 | 87.0 |
| 3 | 60.0 | 23.7 | 16.3 | 59.9 | 21.5 | 18.6 | 55.1 | 27.5 | 17.4 |
| 4 | 44.1 | 16.9 | 39.0 | 37.0 | 18.0 | 45.0 | 47.3 | 36.1 | 16.6 |
| 5 | 37.6 | 12.6 | 49.8 | 36.4 | 19.7 | 43.9 | 16.8 | 34.7 | 48.5 |
| 6 | 15.1 | 49.1 | 35.8 | 10.7 | 42.4 | 46.9 | 10.6 | 42.9 | 46.5 |
| 7 | — | 62.4 | 37.6 | — | 48.0 | 52.0 | — | 52.1 | 47.9 |
| 8 | 16.4 | 30.0 | 53.6 | 8.4 | 24.9 | 66.7 | 14.7 | 32.5 | 52.8 |
| 9 | — | 19.5 | 80.4 | — | 8.5 | 91.5 | — | 13.2 | 86.8 |
| Normal individuals | |||||||||
| 1 | 98.9 | — | — | 99.0 | — | — | 98.4 | — | — |
| 2 | 98.7 | — | — | 98.9 | — | — | 98.4 | — | — |
| 3 | 98.1 | — | — | 98.3 | — | — | 97.5 | — | — |
| 4 | 99.0 | — | — | 99.1 | — | — | 98.9 | — | — |