Table 3. Number of BFU-E Colonies... | American Society of Hematology

Table 3.

Number of BFU-E Colonies Obtained by Erythropoietic Cell Culture, Morphologic Features of Cultured Erythroblasts, and Percentage of Apoptotic Erythroblasts on TUNEL Analysis

Case No.	No. of BFU-E Colonies (/1.0 × 10⁵ cells)^3-150	Morphologic Findings (%)			Apoptotic Erythroblasts (%)
		BAS	POLY	ORTH
PNH patients
1	51, 48, 52	3.0	33.0	64.0	2.6, 3.1
2	73, 69, 76	1.0	42.5	56.5	3.2, 3.5^3-151
3	9, 7, 12	0.0	18.5	81.5	1.8, 2.0
4	4, 2, 3	0.0	52.0	48.0	NT
5	62, 58, 59	1.0	21.5	77.5	NT
6	14, 13, 10	4.0	28.0	68.0	3.3, 3.6^3-151
7	61, 70, 63	0.0	40.5	59.5	1.4, 1.8
8	22, 19, 20	2.0	10.0	88.0	1.1, 1.5
9	23, 16, 18	0.0	39.5	60.5	NT
Normal individuals
1	48, 62, 53	3.6	58.2	38.2	0.6, 0.6
2	54, 56, 52	2.0	50.0	48.0	1.6, 1.8
3	55, 63, 50	0.0	72.0	28.0	1.9, 1.5
4	60, 48, 54	1.4	48.0	50.6	1.3, 1.2

Case No.	No. of BFU-E Colonies (/1.0 × 10⁵ cells)^3-150	Morphologic Findings (%)			Apoptotic Erythroblasts (%)
		BAS	POLY	ORTH
PNH patients
1	51, 48, 52	3.0	33.0	64.0	2.6, 3.1
2	73, 69, 76	1.0	42.5	56.5	3.2, 3.5^3-151
3	9, 7, 12	0.0	18.5	81.5	1.8, 2.0
4	4, 2, 3	0.0	52.0	48.0	NT
5	62, 58, 59	1.0	21.5	77.5	NT
6	14, 13, 10	4.0	28.0	68.0	3.3, 3.6^3-151
7	61, 70, 63	0.0	40.5	59.5	1.4, 1.8
8	22, 19, 20	2.0	10.0	88.0	1.1, 1.5
9	23, 16, 18	0.0	39.5	60.5	NT
Normal individuals
1	48, 62, 53	3.6	58.2	38.2	0.6, 0.6
2	54, 56, 52	2.0	50.0	48.0	1.6, 1.8
3	55, 63, 50	0.0	72.0	28.0	1.9, 1.5
4	60, 48, 54	1.4	48.0	50.6	1.3, 1.2

Abbreviations: BAS, basochromatophilic erythroblasts; POLY, polychromatophilic erythroblasts; ORTH, orthochromatophilic erythroblasts; NT, not tested.

F3-150

In the normal individuals, 3 × 10⁵ peripheral blood mononuclear cells were used.

F3-151

P < .05 v normal individuals by Student's t test.

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