Effect of Nucleoside Analogs on Deoxynucleotides in CCRF-CEM Cells
| Agents . | Deoxynucleotides ( μmol/L) . | |||
|---|---|---|---|---|
| . | dATP . | dCTP . | dGTP . | dTTP . |
| Control | 78.0 ± 6.8 | 13.6 ± 1.3 | 14.8 ± 1.5 | 58.0 ± 12.3 |
| ara-C (0.6 nmol/L) | 61.6 ± 3.3 | 11.5 ± 1.8 | 12.7 ± 5.6 | 46.4 ± 4.0 |
| dFdC (0.8 nmol/L) | 33.8 ± 5.5* | 8.3 ± 0.6* | 9.2 ± 1.6* | 24.2 ± 6.7* |
| F-ara-A (120 nmol/L) | 27.1 ± 4.4* | 7.3 ± 0.6* | 9.7 ± 1.9* | 28.1 ± 7.4* |
| Hydroxyurea (0.2 mmol/L) | 4.7 ± 0.5* | 7.6 ± 1.0* | 5.7 ± 1.5* | 11.4 ± 4.2* |
| Agents . | Deoxynucleotides ( μmol/L) . | |||
|---|---|---|---|---|
| . | dATP . | dCTP . | dGTP . | dTTP . |
| Control | 78.0 ± 6.8 | 13.6 ± 1.3 | 14.8 ± 1.5 | 58.0 ± 12.3 |
| ara-C (0.6 nmol/L) | 61.6 ± 3.3 | 11.5 ± 1.8 | 12.7 ± 5.6 | 46.4 ± 4.0 |
| dFdC (0.8 nmol/L) | 33.8 ± 5.5* | 8.3 ± 0.6* | 9.2 ± 1.6* | 24.2 ± 6.7* |
| F-ara-A (120 nmol/L) | 27.1 ± 4.4* | 7.3 ± 0.6* | 9.7 ± 1.9* | 28.1 ± 7.4* |
| Hydroxyurea (0.2 mmol/L) | 4.7 ± 0.5* | 7.6 ± 1.0* | 5.7 ± 1.5* | 11.4 ± 4.2* |
CCRF-CEM cells were incubated for 13 hours with 0.6 nmol/L ara-C, 0.8 nmol/L dFdC, 120 nmol/L F-ara-A, or 0.2 mmol/L hydroxyurea. Intracellular dNTP concentrations were determined as indicated in Materials and Methods.
Values are mean ± SD (n = 3) (*P < .01 by Student's t-test).