Effect of sin-1 Plus Anti–HLA-DR MoAb H81.9 on CFU-GM Production in Canine and Human LTMCS
| LTMC (wk of CFU-GM Assay) . | Culture Conditions . | |||
|---|---|---|---|---|
| . | Control . | H81.9* . | H81.9* + Sin-1 (1 μg/mL) . | H81.9* + Sin-1 (100 μg/mL) . |
| (A)Canine | ||||
| 1 | 100 | 24 ± 8 | ND | 12 ± 6 |
| 2 | 100 | 13 ± 7 | ND | 11 ± 7 |
| 3 | 100 | 45 ± 5 | ND | 21 ± 9 |
| 4 | 100 | 30 ± 6 | ND | 17 ± 7 |
| (B)Human | ||||
| 1 | 100 | 32 ± 4 | 51 ± 5 | 18 ± 3 |
| 2 | 100 | 10 ± 7 | 21 ± 6 | 1 ± 1 |
| 3 | 100 | 2 ± 4 | 20 ± 3 | 1 ± 1 |
| 4 | 100 | 0 ± 1 | 18 ± 4 | 0 ± 1 |
| LTMC (wk of CFU-GM Assay) . | Culture Conditions . | |||
|---|---|---|---|---|
| . | Control . | H81.9* . | H81.9* + Sin-1 (1 μg/mL) . | H81.9* + Sin-1 (100 μg/mL) . |
| (A)Canine | ||||
| 1 | 100 | 24 ± 8 | ND | 12 ± 6 |
| 2 | 100 | 13 ± 7 | ND | 11 ± 7 |
| 3 | 100 | 45 ± 5 | ND | 21 ± 9 |
| 4 | 100 | 30 ± 6 | ND | 17 ± 7 |
| (B)Human | ||||
| 1 | 100 | 32 ± 4 | 51 ± 5 | 18 ± 3 |
| 2 | 100 | 10 ± 7 | 21 ± 6 | 1 ± 1 |
| 3 | 100 | 2 ± 4 | 20 ± 3 | 1 ± 1 |
| 4 | 100 | 0 ± 1 | 18 ± 4 | 0 ± 1 |
Results summarize data from two experiments each for canine and human cells. At each timepoint CFU-GM assays were set up in triplicate. Results are expressed as percent of control. Colony numbers in canine control cultures were 123 ± 14, 81 ± 12, 79 ± 15, and 62 ± 18, and in human cultures 240 ± 28, 215 ± 30, 180 ± 27, and 160 ± 36 at 1, 2, 3, and 4 weeks, respectively.
Abbreviation: ND, not done.
10 μg/mL in all assays.