PCR Primers and Probes Used for EBV Detection and Expression Analysis
| Primers/Probes . | B95.8 Coordinates . | Sequence (bp) . | Annealing Temperature (°C) . | MgCl2 Concentration (mmol/L) . |
|---|---|---|---|---|
| . | (5′-3′ ) . | . | . | . |
| DNA-PCR: | ||||
| EBNA2 5′ | 48162-48181 | 5′-AGGCTGCCCACCCTGAGGAT-3′ | 62 | 1.5 |
| EBNA2 3′ | 48330-48311 | 5′-GCCACCTGGCAGCCCTAAAG-3′ | ||
| EBNA2-type 1/2 probe | 48253-48271 | 5′-GTTGCCGCCAGGTGGCAGC-3′ | ||
| EBNA3C 5′ | 99939-99958 | 5′-AGAAGGGGAGCGTGTGTTGT-3′ | 58 | 1.5 |
| EBNA3C 3′ | 100091-100072 | 5′-GGCTCGTTTTTGACGTCGGC-3′ | ||
| EBNA3C-type 1 probe | 100002-100021 | 5′-GAAGATTCATCGTCAGTGTC-3′ | ||
| EBNA3C-type 2 probe | 5′-CCGTGATTTCTACCGGGAGT-3′ | |||
| RT-PCR: | ||||
| EBNA2 5′ | 47940-47959 | 5′-GCGCCAATCTGTCTACATAG-3′ | 50 | 0.5 |
| EBNA2 3′ | 48534-48515 | 5′-CCCCATGTAACGCAAGATAG-3′ | ||
| EBNA2 probe | 48473-48454 | 5′-CGGGTGCTTAGAAGGTTGTT-3′ | ||
| LMP1 5′ | 168813-168833 | 5′-CAACCAATAGAGTCCACCAGT-3′ | 50 | 0.75 |
| LMP1 3′ | 169263-169243 | 5′-TCTTCAGAAGAGACCTTCTCT-3′ | ||
| LMP1 probe | 168888-168908 | 5′-GGAAGAAGGCCAAAAGCTGCC-3′ | ||
| LMP2A 5′ | 166820-166840 | 5′-GATTGCAACACGACGGGAATG-3′ | 50 | 1.0 |
| LMP2B 5′ | 169819-169839 | 5′-CAGTGTAATCTGCACAAAGAG-3′ | 50 | 0.5 |
| LMP2A/LMP2B 3′ | 271-251 | 5′-AAGTGACAACCGCAGTAAGCA-3′ | ||
| LMP2A/LMP2B probe | 229-249 | 5′-AAACTGCTGACACCGGTGACA-3′ | ||
| BZLF 5′ | 102478-102498 | 5′-GCCACCCGATTCTTGTATCGC-3′ | 55 | 1.5 |
| BZLF 3′ | 103063-103042 | 5′-AGTCTATCAGGACCTGGGAGGG-3′ | ||
| BZLF probe | 102853-102873 | 5′-TTCCCAGTCTCCGACATAACC-3′ |
| Primers/Probes . | B95.8 Coordinates . | Sequence (bp) . | Annealing Temperature (°C) . | MgCl2 Concentration (mmol/L) . |
|---|---|---|---|---|
| . | (5′-3′ ) . | . | . | . |
| DNA-PCR: | ||||
| EBNA2 5′ | 48162-48181 | 5′-AGGCTGCCCACCCTGAGGAT-3′ | 62 | 1.5 |
| EBNA2 3′ | 48330-48311 | 5′-GCCACCTGGCAGCCCTAAAG-3′ | ||
| EBNA2-type 1/2 probe | 48253-48271 | 5′-GTTGCCGCCAGGTGGCAGC-3′ | ||
| EBNA3C 5′ | 99939-99958 | 5′-AGAAGGGGAGCGTGTGTTGT-3′ | 58 | 1.5 |
| EBNA3C 3′ | 100091-100072 | 5′-GGCTCGTTTTTGACGTCGGC-3′ | ||
| EBNA3C-type 1 probe | 100002-100021 | 5′-GAAGATTCATCGTCAGTGTC-3′ | ||
| EBNA3C-type 2 probe | 5′-CCGTGATTTCTACCGGGAGT-3′ | |||
| RT-PCR: | ||||
| EBNA2 5′ | 47940-47959 | 5′-GCGCCAATCTGTCTACATAG-3′ | 50 | 0.5 |
| EBNA2 3′ | 48534-48515 | 5′-CCCCATGTAACGCAAGATAG-3′ | ||
| EBNA2 probe | 48473-48454 | 5′-CGGGTGCTTAGAAGGTTGTT-3′ | ||
| LMP1 5′ | 168813-168833 | 5′-CAACCAATAGAGTCCACCAGT-3′ | 50 | 0.75 |
| LMP1 3′ | 169263-169243 | 5′-TCTTCAGAAGAGACCTTCTCT-3′ | ||
| LMP1 probe | 168888-168908 | 5′-GGAAGAAGGCCAAAAGCTGCC-3′ | ||
| LMP2A 5′ | 166820-166840 | 5′-GATTGCAACACGACGGGAATG-3′ | 50 | 1.0 |
| LMP2B 5′ | 169819-169839 | 5′-CAGTGTAATCTGCACAAAGAG-3′ | 50 | 0.5 |
| LMP2A/LMP2B 3′ | 271-251 | 5′-AAGTGACAACCGCAGTAAGCA-3′ | ||
| LMP2A/LMP2B probe | 229-249 | 5′-AAACTGCTGACACCGGTGACA-3′ | ||
| BZLF 5′ | 102478-102498 | 5′-GCCACCCGATTCTTGTATCGC-3′ | 55 | 1.5 |
| BZLF 3′ | 103063-103042 | 5′-AGTCTATCAGGACCTGGGAGGG-3′ | ||
| BZLF probe | 102853-102873 | 5′-TTCCCAGTCTCCGACATAACC-3′ |