Molecular Profile of EBV in HIV-1–Related Lymphadenopathies
| Patient Code . | Ig (SB) . | Ig (PCR) . | EBV (SB) . | EBV Type (PCR) . | EBV DNA Amount (PCR)3-150 . | LMP . | |
|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | No. Variants . | No. Repeats3-151 . |
| 1 | G | Polyclonal | − | 1 | 2+ | 1 | 4 |
| 2 | G | Polyclonal | − | 1/2 | 1+/1+ | 2 | 4, 3 |
| 3 | G | Polyclonal | − | 1 | 3+ | 1 | 5 |
| 4 | G | Polyclonal | − | 1/2 | 1+/2+ | 1 | 4 |
| 5 | G | Polyclonal | − | − | − | − | − |
| 6 | G | Polyclonal | − | NT | 2+ | 2 | 4, 5 |
| 7 | G | Polyclonal | − | 1 | 1+ | 1 | 5 |
| 8 | G | Polyclonal | − | 1 | 1+ | 1 | 5 |
| 9 | G | Polyclonal | − | 1 | 1+ | 1 | 5 |
| 10 | G | Polyclonal | − | 1 | 1+ | 3 | 4, 5, 6 |
| 11 | G | Polyclonal | − | 1 | 2+ | 1 | 3 |
| 12 | G | Polyclonal | − | 1 | 1+ | 1 | 5 |
| 13 | G | Polyclonal | − | 1/2 | 1+/1+ | 2 | 4, 3 |
| 14 | G | Polyclonal | − | − | − | − | − |
| 15 | NA | Polyclonal | NA | − | − | − | − |
| 16 | NA | Polyclonal | NA | 1 | 2+ | 1 | 4 |
| 17 | G | Polyclonal | − | 1 | 2+ | 2 | 5, 5 |
| 18 | G | Polyclonal | − | 1 | 1+ | 2 | 7, 5 |
| 19 | G | ND | − | 1/2 | 1+/1+ | 2 | 4, 7 |
| 20 | G | Polyclonal | − | 1 | 2+ | 2 | 4, 5 |
| 21 | G | Polyclonal | − | 1 | 1+ | 2 | 4, 5 |
| 22 | ND | ND | ND | 1 | 1+ | ND | ND |
| 23 | G | Polyclonal | − | 1/2 | 2+/1+ | 1 | 4 |
| 24 | G | Polyclonal | − | 1/2 | 3+/3+ | 2 | 4, 7 |
| 25 | NA | Polyclonal | NA | 2 | 1+ | 1 | 5 |
| 26 | G | Polyclonal | — | 1 | 1+ | 1 | 4 |
| 27 | NA | Polyclonal | NA | 1 | 2+ | 1 | 5 |
| 28 | NA | Polyclonal | NA | 1 | 1+ | 1 | 5 |
| Patient Code . | Ig (SB) . | Ig (PCR) . | EBV (SB) . | EBV Type (PCR) . | EBV DNA Amount (PCR)3-150 . | LMP . | |
|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | No. Variants . | No. Repeats3-151 . |
| 1 | G | Polyclonal | − | 1 | 2+ | 1 | 4 |
| 2 | G | Polyclonal | − | 1/2 | 1+/1+ | 2 | 4, 3 |
| 3 | G | Polyclonal | − | 1 | 3+ | 1 | 5 |
| 4 | G | Polyclonal | − | 1/2 | 1+/2+ | 1 | 4 |
| 5 | G | Polyclonal | − | − | − | − | − |
| 6 | G | Polyclonal | − | NT | 2+ | 2 | 4, 5 |
| 7 | G | Polyclonal | − | 1 | 1+ | 1 | 5 |
| 8 | G | Polyclonal | − | 1 | 1+ | 1 | 5 |
| 9 | G | Polyclonal | − | 1 | 1+ | 1 | 5 |
| 10 | G | Polyclonal | − | 1 | 1+ | 3 | 4, 5, 6 |
| 11 | G | Polyclonal | − | 1 | 2+ | 1 | 3 |
| 12 | G | Polyclonal | − | 1 | 1+ | 1 | 5 |
| 13 | G | Polyclonal | − | 1/2 | 1+/1+ | 2 | 4, 3 |
| 14 | G | Polyclonal | − | − | − | − | − |
| 15 | NA | Polyclonal | NA | − | − | − | − |
| 16 | NA | Polyclonal | NA | 1 | 2+ | 1 | 4 |
| 17 | G | Polyclonal | − | 1 | 2+ | 2 | 5, 5 |
| 18 | G | Polyclonal | − | 1 | 1+ | 2 | 7, 5 |
| 19 | G | ND | − | 1/2 | 1+/1+ | 2 | 4, 7 |
| 20 | G | Polyclonal | − | 1 | 2+ | 2 | 4, 5 |
| 21 | G | Polyclonal | − | 1 | 1+ | 2 | 4, 5 |
| 22 | ND | ND | ND | 1 | 1+ | ND | ND |
| 23 | G | Polyclonal | − | 1/2 | 2+/1+ | 1 | 4 |
| 24 | G | Polyclonal | − | 1/2 | 3+/3+ | 2 | 4, 7 |
| 25 | NA | Polyclonal | NA | 2 | 1+ | 1 | 5 |
| 26 | G | Polyclonal | — | 1 | 1+ | 1 | 4 |
| 27 | NA | Polyclonal | NA | 1 | 2+ | 1 | 5 |
| 28 | NA | Polyclonal | NA | 1 | 1+ | 1 | 5 |
Abbreviations: ND, not done; NA, not applicable because only low-molecular-weight DNA was available; NT, not typable.
The relative amount of EBV DNA was estimated by comparing OD values of amplified products to those obtained by amplification of 10-fold serial dilutions of B95.8 or AG876 DNA: 1+ (0.1-1 pg), 2+ (1-10 pg), 3+ (10-100 pg).
In boldface are reported LMP1 variants containing the 15-bp insertion in the third repeat element.