Molecular Profile of EBV in HIV-1–Related NHLs
| Patient Code . | Site . | Pathology . | Ig (SB) . | Ig (PCR) . | EBV (SB)4-150 . | EBER (ISH)4-151 . | EBV Type (PCR) . | EBV DNA Amount (PCR)‡ . | LMP . | |
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | . | . | No. Variants . | No. Repeatsρ . |
| 29 | Lymph node | SNCCL | R | Clonal | + | ND | 2 | 5+ | 1 | 4 |
| 30 | Lymph node | SNCCL | R | Clonal | + | 20% | 1 | 6+ | 1 | 5 |
| 31 | Bone marrow | SNCCL | ND | ND | − | ND | − | − | − | − |
| 32 | Stomach | SNCCL | NA | Clonal | NA | 10% | 2 | 4+ | 1 | 3 |
| 33 | Ascites | SNCCL | R | Clonal | + | ND | 2 | 5+ | 1 | 6 |
| 34 | Brain | CNS-LCL | R | Clonal | + | ND | 1 | 5+ | 1 | 6 |
| 35 | Brain | CNS-LCL | NA | Clonal | NA | ND | − | − | − | − |
| 36 | Brain | CNS-LCL | NA | Clonal | NA | 15% | 1 | 4+ | ND | ND |
| 37 | Lymph node | LCL-IB | R | Clonal | − | ND | 1 | 3+ | 1 | 4 |
| 38 | Stomach | LCL-IB | ND | ND | ND | ND | 1 | 1+ | ND | ND |
| 39 | Lymph node | LCL-IBP | G | Polyclonal | − | ND | − | − | − | − |
| 40 | Skin | LNCCL | NA | Clonal | NA | 30% | 1 | 1+ | 1 | 4 |
| 41 | Skin | LCL-IBP | NA | Clonal | NA | − | 1 | 2+ | 1 | 4 |
| 42 | Bladder wall | LNCCL | NA | Polyclonal | NA | − | 1/2 | 1+/2+ | 2 | 4, 5 |
| 43 | Lymph node | LNCCL | R | Clonal | − | − | − | − | − | − |
| 44 | Lymph node | LCL-IBP | R | Clonal | + | 45% | 1 | 4+ | 1 | 4 |
| 45 | Lymph node | LNCCL | R | Clonal | + | 20% | 1 | 5+ | 1 | 5 |
| 46 | Stomach | LCL-IB | R | Clonal | + | 45% | 1 | 5+ | 1 | 5 |
| 47 | Nasopharynx | LCL-IB | ND | Clonal | + | ND | 1 | 4+ | 1 | 6 |
| 48 | Pleural effusion | LCL-IB | R | Clonal | − | − | 1 | 3+ | 1 | 4 |
| Patient Code . | Site . | Pathology . | Ig (SB) . | Ig (PCR) . | EBV (SB)4-150 . | EBER (ISH)4-151 . | EBV Type (PCR) . | EBV DNA Amount (PCR)‡ . | LMP . | |
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | . | . | No. Variants . | No. Repeatsρ . |
| 29 | Lymph node | SNCCL | R | Clonal | + | ND | 2 | 5+ | 1 | 4 |
| 30 | Lymph node | SNCCL | R | Clonal | + | 20% | 1 | 6+ | 1 | 5 |
| 31 | Bone marrow | SNCCL | ND | ND | − | ND | − | − | − | − |
| 32 | Stomach | SNCCL | NA | Clonal | NA | 10% | 2 | 4+ | 1 | 3 |
| 33 | Ascites | SNCCL | R | Clonal | + | ND | 2 | 5+ | 1 | 6 |
| 34 | Brain | CNS-LCL | R | Clonal | + | ND | 1 | 5+ | 1 | 6 |
| 35 | Brain | CNS-LCL | NA | Clonal | NA | ND | − | − | − | − |
| 36 | Brain | CNS-LCL | NA | Clonal | NA | 15% | 1 | 4+ | ND | ND |
| 37 | Lymph node | LCL-IB | R | Clonal | − | ND | 1 | 3+ | 1 | 4 |
| 38 | Stomach | LCL-IB | ND | ND | ND | ND | 1 | 1+ | ND | ND |
| 39 | Lymph node | LCL-IBP | G | Polyclonal | − | ND | − | − | − | − |
| 40 | Skin | LNCCL | NA | Clonal | NA | 30% | 1 | 1+ | 1 | 4 |
| 41 | Skin | LCL-IBP | NA | Clonal | NA | − | 1 | 2+ | 1 | 4 |
| 42 | Bladder wall | LNCCL | NA | Polyclonal | NA | − | 1/2 | 1+/2+ | 2 | 4, 5 |
| 43 | Lymph node | LNCCL | R | Clonal | − | − | − | − | − | − |
| 44 | Lymph node | LCL-IBP | R | Clonal | + | 45% | 1 | 4+ | 1 | 4 |
| 45 | Lymph node | LNCCL | R | Clonal | + | 20% | 1 | 5+ | 1 | 5 |
| 46 | Stomach | LCL-IB | R | Clonal | + | 45% | 1 | 5+ | 1 | 5 |
| 47 | Nasopharynx | LCL-IB | ND | Clonal | + | ND | 1 | 4+ | 1 | 6 |
| 48 | Pleural effusion | LCL-IB | R | Clonal | − | − | 1 | 3+ | 1 | 4 |
Abbreviations: SNCCL, small noncleaved cell Burkitt-like lymphoma; CNS-LCL, central nervous system-large cell lymphoma; LCL-IB, large cell immunoblastic lymphoma; LCL-IBP, large cell immunoblastic lymphoma with plasmacytoid differentiation; ND, not done; NA, not applicable because only low-molecular-weight DNA was available.
Single genome dominance.
% of positive cells.
The relative amount of EBV DNA was estimated by comparing OD values of amplified products to those obtained by amplification of 10-fold serial dilutions of B95.8 or AG876 DNA: 1+ (0.1-1 pg), 2+ (1-10 pg), 3+ (10-100 pg), 4+ (100 pg-1 ng), 5+ (1-10 ng), 6+ (10-100 ng).
ρ In boldface are reported LMP1 variants containing the 15-bp insertion in the third repeat element.