Table 2.

Effects of IL-6 and/or Dex on Cell Cycle Distribution on Multiple Myeloma Cells

Cells*% Cells in G1 Phase
MediaIL-6Dex (μmol/L)Dex + IL-6
0.010.101.00
OCI-My5 41.5 31.8 42.3 50.2 62.5 34.6 
RPMI-8226 37.5 36.8 37.5 40.3 58.9 54.3 
U-266 67.5 57.7 66.2 84.6 92.3 90.4 
HSS 44.5 43.4 45.3 51.5 63.8 62.7 
S6B45 62.6 59.2 64 83.2 89.4 66.3 
ARH-77 55 57.3 54.2 55.6 57.1 53.3 
JKB 79.3 80.2 78.3 85.9 91.8 90.9 
Patient no. 1 81.5 74.3 85.9 93.3 92.5 77.6 
Patient no. 2 85.1 84.6 90.2 93.6 95.3 90.6 
Patient no. 3 88.7 74.2 89.9 94.2 96.6 90.6 
Patient no. 4 87 82.4 91 92.9 93.3 91 
Patient no. 5 78.2 71.3 90.5 93.7 94.6 82.3 
Cells*% Cells in G1 Phase
MediaIL-6Dex (μmol/L)Dex + IL-6
0.010.101.00
OCI-My5 41.5 31.8 42.3 50.2 62.5 34.6 
RPMI-8226 37.5 36.8 37.5 40.3 58.9 54.3 
U-266 67.5 57.7 66.2 84.6 92.3 90.4 
HSS 44.5 43.4 45.3 51.5 63.8 62.7 
S6B45 62.6 59.2 64 83.2 89.4 66.3 
ARH-77 55 57.3 54.2 55.6 57.1 53.3 
JKB 79.3 80.2 78.3 85.9 91.8 90.9 
Patient no. 1 81.5 74.3 85.9 93.3 92.5 77.6 
Patient no. 2 85.1 84.6 90.2 93.6 95.3 90.6 
Patient no. 3 88.7 74.2 89.9 94.2 96.6 90.6 
Patient no. 4 87 82.4 91 92.9 93.3 91 
Patient no. 5 78.2 71.3 90.5 93.7 94.6 82.3 

Cell cycle distribution was examined by PI staining followed by FACS analysis.

*

Cell lines (2 × 105) and patients' MM cells (1 × 106) were cultured for 16 hours with media alone, IL-6 (50 ng/mL), Dex, or IL-6 (50 ng/mL) + Dex (1 μmol/L).

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