Time-Dependent Effects of INF-γ and/or IL-6 on Cell Cycle Distribution of the Cells of Patient No. 1
| . | Time (h) . | |||||||
|---|---|---|---|---|---|---|---|---|
| . | 0 . | 2 . | 4 . | 8 . | 16 . | 24 . | 36 . | 48 . |
| Media | 80.1 | 79.5 | 79.6 | 80.2 | 81.5 | 82.9 | 83.4 | 83.8 |
| IL-6 (50 ng/mL) | 80.1 | 81.8 | 80.2 | 77.5 | 74.3 | 74.1 | 72 | 73.3 |
| INF-γ (10 ng/mL) | 80.1 | 81.7 | 86.7 | 89.5 | 92.6 | 92.5 | 92.2 | 92 |
| IL-6 (50 ng/mL) + INF-γ (10 ng/mL) | 80.1 | 82.6 | 81.9 | 80.3 | 78.5 | 77.6 | 75.3 | 75.2 |
| . | Time (h) . | |||||||
|---|---|---|---|---|---|---|---|---|
| . | 0 . | 2 . | 4 . | 8 . | 16 . | 24 . | 36 . | 48 . |
| Media | 80.1 | 79.5 | 79.6 | 80.2 | 81.5 | 82.9 | 83.4 | 83.8 |
| IL-6 (50 ng/mL) | 80.1 | 81.8 | 80.2 | 77.5 | 74.3 | 74.1 | 72 | 73.3 |
| INF-γ (10 ng/mL) | 80.1 | 81.7 | 86.7 | 89.5 | 92.6 | 92.5 | 92.2 | 92 |
| IL-6 (50 ng/mL) + INF-γ (10 ng/mL) | 80.1 | 82.6 | 81.9 | 80.3 | 78.5 | 77.6 | 75.3 | 75.2 |
Patient no. 1 MM cells (5 × 105) were cultured with 10% FBS RPMI-1640 media alone, IL-6, Dex, or IL-6 + Dex. Cells (1 × 106) were harvested at intervals, washed, stained with PI, and analyzed using FACS. Values are the percentage of cells in G1 phase.