Effect of Heparin on Adhesion of Erythroid Progenitors to Heparin Binding Fragments of FN
| Substrate . | % Adhesion . | |||
|---|---|---|---|---|
| . | Heparin . | CFU-E . | Mature BFU-E . | Primitive BFU-E . |
| BSA | − | 6 ± 1 | <1 | <1 |
| n = 19 | n = 21 | n = 21 | ||
| 30/35 kD | − | 86 ± 3 | 66 ± 3 | 64 ± 4 |
| n = 9 | n = 17 | n = 14 | ||
| + | 85 ± 3 | 37 ± 4* | 44 ± 4* | |
| n = 9 | n = 17 | n = 14 | ||
| 42 kD | − | 65 ± 4 | 48 ± 3 | 44 ± 3 |
| n = 5 | n = 5 | n = 5 | ||
| + | 13 ± 3* | 7 ± 2* | <1* | |
| n = 5 | n = 3 | n = 3 | ||
| Substrate . | % Adhesion . | |||
|---|---|---|---|---|
| . | Heparin . | CFU-E . | Mature BFU-E . | Primitive BFU-E . |
| BSA | − | 6 ± 1 | <1 | <1 |
| n = 19 | n = 21 | n = 21 | ||
| 30/35 kD | − | 86 ± 3 | 66 ± 3 | 64 ± 4 |
| n = 9 | n = 17 | n = 14 | ||
| + | 85 ± 3 | 37 ± 4* | 44 ± 4* | |
| n = 9 | n = 17 | n = 14 | ||
| 42 kD | − | 65 ± 4 | 48 ± 3 | 44 ± 3 |
| n = 5 | n = 5 | n = 5 | ||
| + | 13 ± 3* | 7 ± 2* | <1* | |
| n = 5 | n = 3 | n = 3 | ||
Bone marrow cells were incubated in wells coated with untreated and heparin-treated 30-/35- and 42-kD heparin binding fragments. Unattached and attached populations were then assayed for the presence of eyrthroid progenitors. The results were expressed as the mean percent cell adhesion ± SEM.
P ≤ .002.