Clonogenic Progenitors Differential in Peripheral Blood of Animals Treated With FL, G-CSF, or G-CSF Plus FL
| . | Clonogenic Progenitors* . | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | BFU-E . | GM-CFU . | HPP-CFC . | CFU-Mk . | CFU-L . | O-CFC† . | . | . | . | . | . | . | ||||||
| . | No./105 . | No./mL . | No./105 . | No./mL . | No./105 . | No./mL . | No./105 . | No./mL . | No./105 . | No./mL . | No./105 . | No./mL . | . | . | . | . | . | . |
| FL (2)‡ | 1.3 ± 0.5 | 169.4 ± 1.9 | 60.3 ± 17 | 5,595 ± 1,472 | 0.54 ± 0.54 | 46.5 ± 46.5 | 1.8 | 182 | 7.1 ± 3.1 | 664 ± 320 | ||||||||
| G-CSF (2) | 1.75 ± 0.75 | 467 ± 203 | 33 ± 18 | 8,770 ± 4,847 | 1.10 ± 0.4 | 388 ± 106 | 2.6 ± 2.0 | 742 ± 503 | 6.0 ± 2.0 | 2,199 ± 695 | 0 | 0 | ||||||
| FL + G-CSF (3) | 24.4 ± 17 | 7,689 ± 3,751 | 111 ± 22 | 40,910 ± 16,014 | 5.4 ± 0.6 | 4,213 ± 1,391 | 12 ± 9.0 | 2,122 ± 576 | 18.0 ± 11.0 | 3,737 ± 1,296 | 80 ± 70 | 26,690 ± 19,961 | ||||||
| . | Clonogenic Progenitors* . | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | BFU-E . | GM-CFU . | HPP-CFC . | CFU-Mk . | CFU-L . | O-CFC† . | . | . | . | . | . | . | ||||||
| . | No./105 . | No./mL . | No./105 . | No./mL . | No./105 . | No./mL . | No./105 . | No./mL . | No./105 . | No./mL . | No./105 . | No./mL . | . | . | . | . | . | . |
| FL (2)‡ | 1.3 ± 0.5 | 169.4 ± 1.9 | 60.3 ± 17 | 5,595 ± 1,472 | 0.54 ± 0.54 | 46.5 ± 46.5 | 1.8 | 182 | 7.1 ± 3.1 | 664 ± 320 | ||||||||
| G-CSF (2) | 1.75 ± 0.75 | 467 ± 203 | 33 ± 18 | 8,770 ± 4,847 | 1.10 ± 0.4 | 388 ± 106 | 2.6 ± 2.0 | 742 ± 503 | 6.0 ± 2.0 | 2,199 ± 695 | 0 | 0 | ||||||
| FL + G-CSF (3) | 24.4 ± 17 | 7,689 ± 3,751 | 111 ± 22 | 40,910 ± 16,014 | 5.4 ± 0.6 | 4,213 ± 1,391 | 12 ± 9.0 | 2,122 ± 576 | 18.0 ± 11.0 | 3,737 ± 1,296 | 80 ± 70 | 26,690 ± 19,961 | ||||||
Abbreviations: BFU-E, erythroid burst-forming unit; GM-CFU, granulocyte macrophage colony-forming unit; HPP-CFC, high proliferative potential-colony-forming cell; CFU-Mk, megakaryocyte colony-forming unit; CFU-L, lymphocyte colony-forming unit; O-CFC, osteoclastic colony-forming cell; FL, Flt3 ligand; G-CSF, granulocyte colony-stimulating factor.
At peak level.
Osteoclastic colonies were those over 8 cells each and were cultured with supernate from SJ3 cell line, as described.24 These progenitors are not included in calculations presented in Figs 1-3.
Number in parenthesis indicates numbers of animals in which the detailed progenitor differential was studied.