T-Cell Precursor Frequencies in Mixed Lymphocyte Culture Responses
| HCL Patient . | HTLp . | PTLp . | CTLp . | CTLp . | Relation to RT-PCR Data . |
|---|---|---|---|---|---|
| Day of IFN Therapy . | per 106T Cells . | per 106 T Cells . | Allogeneic Cells . | Autologous Cells . | . |
| A IFN d 28* | 9 | 11 | 280 | 43 | By PCR good improvement of skewed T-cell repertoire |
| A IFN d 1,183 | 109 | 198 | 935 | 26 | |
| B IFN d −221* | 15 | 16 | 16 | 3 | By PCR good improvement of skewed T-cell repertoire |
| B IFN d 1,062 | 511 | 205 | 142 | 68 | |
| Against 1st donor | |||||
| - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - | |||||
| idem IFN d −221 | 17 | 45 | 12 | 0 | |
| idem IFN d 1,062 | 63 | 132 | 16 | 0 | |
| Against 2nd donor | |||||
| G IFN d 0 | 48 | 85 | 13 | 0 | By PCR only minor improvement of T-cell repertoire |
| G IFN d 1,050 | 61 | 54 | 9 | 0 | |
| Against 1st donor | |||||
| - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - | |||||
| idem IFN d 0 | 80 | 33 | 26 | 7 | |
| idem IFN d 1,050 | 18 | 13 | 17 | 0 | |
| Against 2nd donor | |||||
| HCL Patient . | HTLp . | PTLp . | CTLp . | CTLp . | Relation to RT-PCR Data . |
|---|---|---|---|---|---|
| Day of IFN Therapy . | per 106T Cells . | per 106 T Cells . | Allogeneic Cells . | Autologous Cells . | . |
| A IFN d 28* | 9 | 11 | 280 | 43 | By PCR good improvement of skewed T-cell repertoire |
| A IFN d 1,183 | 109 | 198 | 935 | 26 | |
| B IFN d −221* | 15 | 16 | 16 | 3 | By PCR good improvement of skewed T-cell repertoire |
| B IFN d 1,062 | 511 | 205 | 142 | 68 | |
| Against 1st donor | |||||
| - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - | |||||
| idem IFN d −221 | 17 | 45 | 12 | 0 | |
| idem IFN d 1,062 | 63 | 132 | 16 | 0 | |
| Against 2nd donor | |||||
| G IFN d 0 | 48 | 85 | 13 | 0 | By PCR only minor improvement of T-cell repertoire |
| G IFN d 1,050 | 61 | 54 | 9 | 0 | |
| Against 1st donor | |||||
| - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - | |||||
| idem IFN d 0 | 80 | 33 | 26 | 7 | |
| idem IFN d 1,050 | 18 | 13 | 17 | 0 | |
| Against 2nd donor | |||||
*Time point in relation to start of IFN-α treatment. Case A still showed many TCRBV abnormalities at day 28, and the cells collected at that time were therefore considered suitable for this analysis. Cells from Case B were collected 7 months before start of treatment.