Table 2. Sequence and Nucleotide... | American Society of Hematology

Table 2.

Sequence and Nucleotide Position of the Primers Used in HGV RNA Amplification

		5′ Noncoding Region
		Nucleotide Sequence	Position
Outer primers	S1	CACTGGGTGCAAGCCCCAGAA	13-33
	A1	CACTGGTCCTTGTCAACTCGC	358-378
Inner primers	S2	CGACGCCTACTGAAGTAGACG	36-56
	A2	GTACGCCTATTGGTCAAGAGA	336-356
		NS3/Helicase Region

Outer primers	AMS1	GCTCGCCTATGACTCAGCATC	4194-4214
	AMS3	GTCACCTCAACGACCTCCTCC	4504-4524
Inner primers	AMS2	GAGACAAAGCTGGACGTTGGT	4226-4246
	AMS4	CAACCCACAGTCGGTGACAGA	4478-4498

		5′ Noncoding Region
		Nucleotide Sequence	Position
Outer primers	S1	CACTGGGTGCAAGCCCCAGAA	13-33
	A1	CACTGGTCCTTGTCAACTCGC	358-378
Inner primers	S2	CGACGCCTACTGAAGTAGACG	36-56
	A2	GTACGCCTATTGGTCAAGAGA	336-356
		NS3/Helicase Region

Outer primers	AMS1	GCTCGCCTATGACTCAGCATC	4194-4214
	AMS3	GTCACCTCAACGACCTCCTCC	4504-4524
Inner primers	AMS2	GAGACAAAGCTGGACGTTGGT	4226-4246
	AMS4	CAACCCACAGTCGGTGACAGA	4478-4498