Table 2. Percentage of Cycling Cells in... | American Society of Hematology

Table 2.

Percentage of Cycling Cells in Rh-123 and BodVer Subsets of HSC

	Rh-123^low	Rh-123^high	BodVer^low	BodVer^high
No blocker	3.2 ± 3.5	29.9 ± 4.5	3.0 ± 1.2	30.2 ± 7.7
Verapamil	3.4 ± 1.6	29.1 ± 0.5	4.5^*	32.3^*
Reserpine	2.8 ± 0.7	46.0 ± 14.3	2.2 ± 0.4	54.3 ± 7.4
Cyclosporin A	3.7 ± 1.7	46.9 ± 5.7	3.2 ± 1.4	49.9 ± 3.2

	Rh-123^low	Rh-123^high	BodVer^low	BodVer^high
No blocker	3.2 ± 3.5	29.9 ± 4.5	3.0 ± 1.2	30.2 ± 7.7
Verapamil	3.4 ± 1.6	29.1 ± 0.5	4.5^*	32.3^*
Reserpine	2.8 ± 0.7	46.0 ± 14.3	2.2 ± 0.4	54.3 ± 7.4
Cyclosporin A	3.7 ± 1.7	46.9 ± 5.7	3.2 ± 1.4	49.9 ± 3.2

Thy-1.1^lowSca-1⁺Lin⁻ cells were stained and separated based on levels of Rh-123 and BodVer staining in the absence or presence of the indicated P-gp blockers. In each case, the brightest 15% (Rh-123^low and BodVer^low) of total cells were sorted, and cell cycle analysis was performed for each cell population. Each number represents the percentage of cells in the S/G₂/M regions of the DNA histogram (mean ± SD) as determined in three or four separate sorting experiments except as noted.

Average values from two experiments.

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