Comparative Analysis of Absolute Numbers and Cycling Status of Myeloid Progenitor Cells From Bone Marrow and Spleen ofFac−/− and Wild-Type Littermate Controls
| . | . | . | . | . | Progenitor Suicide Rates . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mice . | Nucleated Cells × 106 . | Progenitors × 103/Organ . | CFU-GM . | BFU-E . | CFU-GEMM . | |||||
| CFU-GM . | BFU-E . | CFU-GEMM . | 3HTdR . | HU . | 3HTdr . | HU . | 3HTdr . | HU . | ||
| Bone marrow | ||||||||||
| +/+ | 21.8 ± 2.1 | 33.2 ± 4.1 | 5.1 ± 1.3 | 1.7 ± 0.3 | 22.0 ± 3.9 | 21.5 ± 2.2 | 16.2 ± 5.1 | 14.7 ± 6.2 | 32.4 ± 5.0 | 29.1 ± 4.7 |
| −/− | 19.3 ± 2.3 | 33.9 ± 6.6 | 7.0 ± 1.9 | 1.5 ± 0.3 | 58.9 ± 3.6 | 68.5 ± 1.4 | 59.0 ± 2.4 | 58.2 ± 2.8 | 69.8 ± 5.0 | 79.9 ± 3.4 |
| (NS) | (NS) | (NS) | (NS) | (<.001) | (<.001) | (<.001) | (<.001) | (<.001) | (<.001) | |
| Spleen | ||||||||||
| +/+ | 119.6 ± 13.3 | 6.7 ± 1.4 | 6.4 ± 1.7 | 0.6 ± 0.1 | 0.2 ± 0.2 | 0 ± 0 | 1.9 ± 1.6 | 0.5 ± 0.3 | 1.6 ± 1.6 | 3.1 ± 3.2 |
| −/− | 94.0 ± 10.6 | 6.5 ± 0.6 | 8.4 ± 2.2 | 0.7 ± 0.2 | 53.5 ± 3.6 | 50.9 ± 8.1 | 54.1 ± 3.0 | 52.4 ± 3.4 | 66.4 ± 4.5 | 70.3 ± 4.4 |
| (NS) | (NS) | (NS) | (NS) | (<.001) | (<.001) | (<.001) | (<.001) | (<.001) | (<.001) | |
| . | . | . | . | . | Progenitor Suicide Rates . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mice . | Nucleated Cells × 106 . | Progenitors × 103/Organ . | CFU-GM . | BFU-E . | CFU-GEMM . | |||||
| CFU-GM . | BFU-E . | CFU-GEMM . | 3HTdR . | HU . | 3HTdr . | HU . | 3HTdr . | HU . | ||
| Bone marrow | ||||||||||
| +/+ | 21.8 ± 2.1 | 33.2 ± 4.1 | 5.1 ± 1.3 | 1.7 ± 0.3 | 22.0 ± 3.9 | 21.5 ± 2.2 | 16.2 ± 5.1 | 14.7 ± 6.2 | 32.4 ± 5.0 | 29.1 ± 4.7 |
| −/− | 19.3 ± 2.3 | 33.9 ± 6.6 | 7.0 ± 1.9 | 1.5 ± 0.3 | 58.9 ± 3.6 | 68.5 ± 1.4 | 59.0 ± 2.4 | 58.2 ± 2.8 | 69.8 ± 5.0 | 79.9 ± 3.4 |
| (NS) | (NS) | (NS) | (NS) | (<.001) | (<.001) | (<.001) | (<.001) | (<.001) | (<.001) | |
| Spleen | ||||||||||
| +/+ | 119.6 ± 13.3 | 6.7 ± 1.4 | 6.4 ± 1.7 | 0.6 ± 0.1 | 0.2 ± 0.2 | 0 ± 0 | 1.9 ± 1.6 | 0.5 ± 0.3 | 1.6 ± 1.6 | 3.1 ± 3.2 |
| −/− | 94.0 ± 10.6 | 6.5 ± 0.6 | 8.4 ± 2.2 | 0.7 ± 0.2 | 53.5 ± 3.6 | 50.9 ± 8.1 | 54.1 ± 3.0 | 52.4 ± 3.4 | 66.4 ± 4.5 | 70.3 ± 4.4 |
| (NS) | (NS) | (NS) | (NS) | (<.001) | (<.001) | (<.001) | (<.001) | (<.001) | (<.001) | |
Results are expressed as mean ±1 SEM for 8 mice per group (from a total of 2 experiments), with each mouse assayed individually, for all values shown except for the progenitor suicide rates determined by hydroxyurea (HU) treatment in which 4 mice per group (from 1 experiment) were each assayed individually. The progenitor suicide rates determined by HU treatment can be compared with those values determined by the high specific activity tritiated thymidine (3THdr) kill assay. P values indicated in parentheses.
Abbreviation: NS, not significant (P > .05).