Table 1. NF-κB Expression in Response to... | American Society of Hematology

Table 1.

NF-κB Expression in Response to GM-CSF Stimulation of TF-1 Cells

	Time (h)
	0	2	4	8	10	16	20	24
p105	0.51 ± 0.17	0.49 ± 0.37	0.36 ± 0.15	0.26 ± 0.07	0.29 ± 0.08	0.55 ± 0.19	0.41 ± 0.07	0.44 ± 0.16
p50	0.47 ± 0.14	0.46 ± 0.23	0.35 ± 0.10	0.28 ± 0.09	0.35 ± 0.09	0.53 ± 0.17	0.50 ± 0.12	0.61 ± 0.23
p100	0.70 ± 0.26	0.88 ± 0.32	0.72 ± 0.27	0.65 ± 0.22	0.62 ± 0.22	0.60 ± 0.25	0.64 ± 0.24	0.73 ± 0.31
p52	0.60 ± 0.21	0.62 ± 0.27	0.53 ± 0.20	0.52 ± 0.22	0.53 ± 0.18	0.94 ± 0.29^-150	1.05 ± 0.24^-150	1.04 ± 0.22^-150
p65	0.98 ± 0.42	1.62 ± 0.78	1.32 ± 0.47	1.12 ± 0.41	1.25 ± 0.47	1.93 ± 0.70	1.63 ± 0.62	1.48 ± 0.70
IκBα	0.66 ± 0.27	0.80 ± 0.30	0.52 ± 0.25	0.25 ± 0.10	0.43 ± 0.20	0.60 ± 0.24	0.56 ± 0.29	0.61 ± 0.24

	Time (h)
	0	2	4	8	10	16	20	24
p105	0.51 ± 0.17	0.49 ± 0.37	0.36 ± 0.15	0.26 ± 0.07	0.29 ± 0.08	0.55 ± 0.19	0.41 ± 0.07	0.44 ± 0.16
p50	0.47 ± 0.14	0.46 ± 0.23	0.35 ± 0.10	0.28 ± 0.09	0.35 ± 0.09	0.53 ± 0.17	0.50 ± 0.12	0.61 ± 0.23
p100	0.70 ± 0.26	0.88 ± 0.32	0.72 ± 0.27	0.65 ± 0.22	0.62 ± 0.22	0.60 ± 0.25	0.64 ± 0.24	0.73 ± 0.31
p52	0.60 ± 0.21	0.62 ± 0.27	0.53 ± 0.20	0.52 ± 0.22	0.53 ± 0.18	0.94 ± 0.29^-150	1.05 ± 0.24^-150	1.04 ± 0.22^-150
p65	0.98 ± 0.42	1.62 ± 0.78	1.32 ± 0.47	1.12 ± 0.41	1.25 ± 0.47	1.93 ± 0.70	1.63 ± 0.62	1.48 ± 0.70
IκBα	0.66 ± 0.27	0.80 ± 0.30	0.52 ± 0.25	0.25 ± 0.10	0.43 ± 0.20	0.60 ± 0.24	0.56 ± 0.29	0.61 ± 0.24

TF-1 cells were deprived of growth factor for 24 hours and then stimulated with 2 ng/mL GM-CSF for 0 to 24 hours. Whole cell lysates were prepared from five experiments and protein from an equivalent cell number was loaded on each lane. Immunoblotting was performed as described in Materials and Methods. The densities of bands were measured with a Molecular Dynamics Densitometer and Quantity One Software, and the mean ± SEM is shown.

F0-150

Significant increase in response to growth factor stimulation (P < .05).

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