Prevention of Rejection by a CTL Clone
| Donor Cells Added . | Percent Donor Cells (day 29) . | |
|---|---|---|
| T Cells . | Granulocytes . | |
| None | 1, 1, 1, 1 | 2, 2, 2, 3 |
| 20 × 106 14C3 CTL | 100, 100, 100, 0 | 99, 97, 98, 2 |
| 20 × 106 3F3 CTL | 1, 1, 1, 1 | 4, 3, 4, 4 |
| 20 × 106 7C11 CTL | 1, 1, 0, 2 | 75, 11, 4, 8 |
| 1.25 × 106 LN T | 100, 100, 100, 100 | 100, 100, 100, 100 |
| Donor Cells Added . | Percent Donor Cells (day 29) . | |
|---|---|---|
| T Cells . | Granulocytes . | |
| None | 1, 1, 1, 1 | 2, 2, 2, 3 |
| 20 × 106 14C3 CTL | 100, 100, 100, 0 | 99, 97, 98, 2 |
| 20 × 106 3F3 CTL | 1, 1, 1, 1 | 4, 3, 4, 4 |
| 20 × 106 7C11 CTL | 1, 1, 0, 2 | 75, 11, 4, 8 |
| 1.25 × 106 LN T | 100, 100, 100, 100 | 100, 100, 100, 100 |
Groups of four irradiated (800 cGy) CB6 recipients were transplanted with 5.0 × 106 T-cell–depleted B6C3 marrow cells with or without freshly isolated lymph node T cells (LN T) or cloned CTL added to the graft. All recipients were treated with IL-2 (30,000 U IP) on the day of transplant and for 6 consecutive days thereafter. On days 29 and 63 after transplantation, the percent of donor T cells and granulocytes in the blood was determined by two-color staining with CD3 and H2KkDk-specific antibodies. Data indicate results for individual recipients tested on day 29. Results of testing on day 63 were similar except that the recipient with 75% donor granulocytes on day 29 after administration of 7C11 cells had only 8% donor granulocytes on day 63 (not shown).